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Merck

B4500

Nα-Benzoyl-L-arginine ethyl ester hydrochloride

trypsin substrate, chromogenic, ≥97% (HPLC), powder

Synonym(s):

BAEE

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About This Item

Empirical Formula (Hill Notation):
C15H22N4O3 · HCl
CAS Number:
2645-08-1
Molecular Weight:
342.82
NACRES:
NA.32
PubChem Substance ID:
24891777
UNSPSC Code:
12352204
EC Number:
220-157-0
MDL number:
MFCD00012579
Beilstein/REAXYS Number:
3781694

Key Documents

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Product Name

Nα-Benzoyl-L-arginine ethyl ester hydrochloride, trypsin substrate

InChI

1S/C15H22N4O3.ClH/c1-2-22-14(21)12(9-6-10-18-15(16)17)19-13(20)11-7-4-3-5-8-11;/h3-5,7-8,12H,2,6,9-10H2,1H3,(H,19,20)(H4,16,17,18);1H/t12-;/m0./s1

InChI key

HIXDELXKSSLIKB-YDALLXLXSA-N

SMILES string

Cl[H].CCOC(=O)[C@H](CCCNC(N)=N)NC(=O)c1ccccc1

assay

≥97% (HPLC)

form

powder

solubility

water: 50 mg/mL, clear, colorless

storage temp.

2-8°C

Quality Level

200

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Application

  • N

  • α-Benzoyl-L-arginine ethyl ester hydrochloride has been used as a substrate:
  • in peptidyl arginine deiminase (PPAD) assay in P. gingivalis vesicles
  • for assaying proteolytic activity of enzymatic extracts of papaya plant parts
  • in trypsin activity assay of the tail tendon fascicles from rat

General description

Nα-Benzoyl-L-arginine ethyl ester hydrochloride is a substrate for trypsin. It is a nonprotein substrate used in spectroscopic assays.
The prototype substrate for trypsin.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000499113
0000499113
BCCN7609
BCCN5707
0000396931

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Ying-Qing Yu et al.
Analytical chemistry, 75(21), 6023-6028 (2003-11-01)
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