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Merck

D9154

o-Dianisidine dihydrochloride

tablet

Synonym(s):

3,3′-Dimethoxybenzidine dihydrochloride, Fast Blue B

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About This Item

Empirical Formula (Hill Notation):
C14H16N2O2 · 2HCl
CAS Number:
20325-40-0
Molecular Weight:
317.21
NACRES:
NA.83
PubChem Substance ID:
24894263
UNSPSC Code:
12352204
EC Number:
243-737-5
MDL number:
MFCD00012488
Beilstein/REAXYS Number:
3917996

Key Documents

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Product Name

o-Dianisidine dihydrochloride, tablet, 10 mg substrate per tablet

InChI

1S/C14H16N2O2.2ClH/c1-17-13-7-9(3-5-11(13)15)10-4-6-12(16)14(8-10)18-2;;/h3-8H,15-16H2,1-2H3;2*1H

InChI key

UXTIAFYTYOEQHV-UHFFFAOYSA-N

SMILES string

Cl.Cl.COc1cc(ccc1N)-c2ccc(N)c(OC)c2

form

tablet

solubility

water: 1 tablet/10 mL, clear, colorless

storage temp.

2-8°C

Quality Level

200

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Biochem/physiol Actions

o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.

Disclaimer

Probably carcinogenic.

Preparation Note

Dissolve one tablet in 60 ml of 50 mM phosphate-citrate buffer, pH 5.0. Add 12 μl of fresh 30% hydrogen peroxide immediately before to use.

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Skin Corr. 1

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

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Certificates of Analysis (COA)

Lot/Batch Number
0000390713
0000390713
SLCJ7840
SLCD6994
SLBX3894

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M Kaszuba et al.
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Reactive cationic and anionic liposomes have been prepared from mixtures of dimyristoylphosphatidylcholine (DMPC) and cholesterol incorporating dimethyldioctadecylammonium bromide and DMPC incorporating phosphatidylinositol, respectively. The liposomes were prepared by the vesicle extrusion technique and had the enzymes glucose oxidase (GO) encapsulated
Frédéric Frottin et al.
Molecular & cellular proteomics : MCP, 5(12), 2336-2349 (2006-09-12)
Methionine aminopeptidase (MAP) is a ubiquitous, essential enzyme involved in protein N-terminal methionine excision. According to the generally accepted cleavage rules for MAP, this enzyme cleaves all proteins with small side chains on the residue in the second position (P1')
Wei Shen et al.
Biosensors & bioelectronics, 44, 171-176 (2013-02-22)
Herein we report a label-free microRNA (miRNA) biosensor in which the formation of a thin insulating film is used to amplify the analytical signal. Briefly, the biosensor is made of an oligonucleotide-coated gold electrode. After hybridizing with a target miRNA
Ozcan Erel
Clinical biochemistry, 38(12), 1103-1111 (2005-10-11)
To develop a new, colorimetric and automated method for measuring total oxidation status (TOS). The assay is based on the oxidation of ferrous ion to ferric ion in the presence of various oxidant species in acidic medium and the measurement
Iain S MacPherson et al.
Protein engineering, design & selection : PEDS, 23(3), 137-145 (2010-01-20)
Directed evolution methods were developed for Cu-containing nitrite reductase (NiR) from Alcaligenes faecalis S-6. The PCR cloning strategy allows for the efficient production of libraries of 100 000 clones by a modification of a megaprimer-based whole-plasmid synthesis reaction. The high-throughput
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