Anti-oligomeric TDP-43

TAR DNA-binding protein 43 (UniProt: Q13148; also known as TDP-43) is encoded by the TARDBP (also known as TDP43) gene (Gene ID: 23435) in human. TDP-43 is a RNA binding protein that is suggested to be involved in various steps of RNA biogenesis and processing and, is mainly present in nucleus. Structurally, it consists of a N-terminal domain (NTD) and two tandem RNA recognition domains (RRM1 and RRM2), followed by a c-terminal glycine-rich region. RRM1 and RRM2 domains are responsible for binding the GU-repeats on RNA molecule. TDP-43 also contains a nuclear localization sequence and a nuclear export sequence, which permits it to transport mRNAs to the cytoplasm and even to synapses as part of neuronal granules. It is hypothesized that TDP-43 forms oligomeric assemblies that associate with tau. Tau oligomers are reported to modulate TDP-43 aggregation state and cellular localization, and TDP-43 aggregates are detected in Alzheimer s disease (AD) brains. Aggregates of post-translationally modified TDP-43 in cytoplasmic inclusion bodies are also characteristic features in frontotemporal dementia (FTD) and amyotrophic lateral sclerosis (ALS). Studies using various biochemical and biophysical assays that examined cross-seeding property of AD, ALS, and FTD brain-derived TDP-43 oligomers (BDT43Os) on tau aggregation suggest that TDP-43/tau interactions may play a role in these neurodegenerative diseases. This rabbit polyclonal antibody is suitable for Western Blotting, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Dot blot applications. (Ref.: Montalbano, M., et al. (2020). Neurobiol. Dis. 146; 105130).

Full-length, recombinant, human TDP-43 treated with hexafluoroisopropanol.

Quality Control Testing

Evaluated by researchers.

Immunoprecipitation Analysis: A representative lot immunoprecipitated oligomeric TDP-43 in Immunoprecipitation application (Montalbano, M., et al. (2020). Neurobiol Dis. 146: 105130).

Western Blotting Analysis: A representative lot detected oligomeric TDP-43 in Western Blotting application (Montalbano, M., et al. (2020). Neurobiol Dis. 146: 105130).

Immunohistochemistry Application: A representative lot detected oligomeric TDP-43 in Immunohistochemistry application (Montalbano, M., et al. (2020). Neurobiol Dis. 146: 105130).

Dot Blot: A representative lot detected oligomeric TDP-43 in Dot Blot application (Montalbano, M., et al. (2020). Neurobiol Dis. 146: 105130).

Immunofluorescence Analysis: A representative lot detected oligomeric TDP-43 in Immunofluorescence application (Montalbano, M., et al. (2020). Neurobiol Dis. 146: 105130).

Filter Trap Assay: A representative lot of this antibody detected oligomeric TDP-43 in Filter Trap Assay. (Montalbano, M., et al. (2020). Neurobiol Dis. 146: 105130).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Anti-oligomeric TDP-43 is a rabbit polyclonal antibody that specifically detects TDP-43 oligomers. This antibody has also been referred as Anti-TDP43-Oligo01 in the literature.

Purified rabbit polyclonal antibody in PBS without preservatives.

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.