Anti-phospho Histone H3 (Thr11) Antibody, clone 6G12C5

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

~17 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

KLH-conjugated linear peptide corresponding to human Histone H3 phosphorylated at Thr11.

This Anti-phospho Histone H3 (Thr11) Antibody, clone 6G12C5 is validated for use in western blotting, dot blot, ELISA & ICC for the detection of phospho Histone H3 (Thr11).

Western Blotting Analysis: 1 µg/mL from a representative lot detected phospho Histone H3 (Thr11) in 10 µg of nocodazole treated HeLa cell lysate.

Dot Blot (Specificity) Analysis: 0.2 µg/mL from a representative lot detected phospho Histone H3 (Thr11) in an Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected phospho Histone H3 (Thr11) in HeLa cells undergoing interphase and metaphase (Prof. Taro Tachibana, Cell Engineering Corporation.).

ELISA Analysis: A representative lot detected phospho Histone H3 (Thr11) in a panel of Histone H3 peptides (Prof. Taro Tachibana, Cell Engineering Corporation.).

Western Blotting Analysis: A representative lot detected phospho Histone H3 (Thr11) in HCT116 cell lysates, which were incubated for a period of time with either nocodazole or UV. The same lot detected phospho Histone H3 (Thr11) in HeLa cell lysates, which were incubated for a period of time with nocodazole. Additionally, the same lot detected phospho Histone H3 (Thr11) in tsFT210 whole cell extracts after released G2/M syncronization (Prof. Taro Tachibana, Cell Engineering Corporation.).

Western Blotting Analysis: A representative lot from an independent laboratory detected phospho Histone H3 (Thr11) in nocodazole treated HeLa extract (Yoshimi, T., et al. (2013). Monoclon Antib Immunodiagn Immunother. 32(2):119-124.)

ELISA Analysis: A representative lot from an independent laboratory detected phospho Histone H3 (Thr11) in a panel of various phosphorylated H3 peptides (Yoshimi, T., et al. (2013). Monoclon Antib Immunodiagn Immunother. 32(2):119-124.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected phospho Histone H3 (Thr11) in mitotic DM4 cells (Yoshimi, T., et al. (2013). Monoclon Antib Immunodiagn Immunother. 32(2):119-124.).

Format: Purified

Evaluated by Western Blotting in nocodazole treated and untreated HeLa acid extract.

Western Blotting Analysis: 1 µg/mL of this antibody detected phospho Histone H3 (Thr11) in 10 µg of nocodazole treated HeLa acid extract.

Replaces: MABE468