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Merck

492016

NP-40 Alternative

Synonym(s):

NP-40 Alternative - CAS 9016-45-9 - Calbiochem, Nonylphenyl Polyethylene Glycol

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About This Item

CAS Number:
9016-45-9
UNSPSC Code:
12161902
NACRES:
NA.25
MDL number:
MFCD00132851

Key Documents

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SMILES string

O(CCOc1ccc(cc1)CCCCCCCCC)CCO

InChI

1S/C19H32O3/c1-2-3-4-5-6-7-8-9-18-10-12-19(13-11-18)22-17-16-21-15-14-20/h10-13,20H,2-9,14-17H2,1H3

InChI key

BLXVTZPGEOGTGG-UHFFFAOYSA-N

description

RTECS - MD0905000

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, desiccated

color

clear colorless

CMC

50 - 300 μM

solubility

water: 25 mg/mL, aqueous buffer: soluble

density

1.06 g/mL

application(s)

(Isolating membrane proteins)

shipped in

ambient

storage temp.

15-25°C

Quality Level

200

Related Categories

General description

A non-ionic surfactant used in the isolation and purification of functional membrane protein complexes and in two-dimensional (2D) gel electrophoresis. Similar in usage to NP-40 and TRITON™ X-100 Detergent(Cat. No. 648462).

Application

NP-40 Alternative has been used:
  • as a component of the lysis buffer for solubilizing proteins from transfected HEK293 cells.
  • as a component of the wash buffer for efficient solubilization of biotinylated plasma proteins in plasma protein profiling and chromatin immunoprecipitation (ChIP).
  • for the purification of doublet microtubules from cilia for single particle cryo-electron microscopy (cryo-EM).

Other Notes

Ripoche, P., et al. 1982. Biochim. Biophys. Acta693, 497.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Disclaimer

Toxicity: Irritant (B)

pictograms

Exclamation markEnvironment
GHS07,GHS09

Storage Class

11 - Combustible Solids

flash_point_f

503.6 °F - Pensky-Martens closed cup - (own results)

flash_point_c

262 °C - Pensky-Martens closed cup - (own results)

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Irrit. 2 - Skin Irrit. 2

Certificates of Analysis (COA)

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Gustav Roder et al.
European journal of immunology, 39(10), 2682-2694 (2009-09-04)
Tapasin (Tpn) is an ER chaperone that is uniquely dedicated to MHC-I biosynthesis. It binds MHC-I molecules, integrates them into peptide-loading complexes, and exerts quality control of the bound peptides; only when an "optimal peptide" is bound will the MHC-I
Ju Gao et al.
Autophagy, 12(4), 632-647 (2016-04-07)
During proteasomal stress, cells can alleviate the accumulation of polyubiquitinated proteins by targeting them to perinuclear aggresomes for autophagic degradation, but the mechanism underlying the activation of this compensatory pathway remains unclear. Here we report that PINK1-s, a short form
Djihad Hadjadj et al.
Aging, 9(12), 2695-2716 (2017-12-29)
High proliferation rate and high mutation density are both indicators of poor prognosis in adrenocortical carcinomas. We performed a hypothesis-driven association study between clinical features in adrenocortical carcinomas and the expression levels of 136 genes involved in DNA metabolism and
Deyi Zhang et al.
Autophagy, 12(9), 1447-1459 (2016-06-16)
Macroautophagy/autophagy is a conserved catabolic process that recycles cytoplasmic material during low energy conditions. BECN1/Beclin1 (Beclin 1, autophagy related) is an essential protein for function of the class 3 phosphatidylinositol 3-kinase (PtdIns3K) complexes that play a key role in autophagy
Chenliang Zhang et al.
Journal of cell science, 131(14) (2018-06-23)
Aggresome formation is a major strategy to enable cells to cope with proteasomal stress. Misfolded proteins are assembled into micro-aggregates and transported to the microtubule organizing center (MTOC) to form perinuclear aggresomes before their degradation through autophagy. So far, multiple
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