H7033
PerfectHyb™ Plus Hybridization Buffer
for Northern and Southern blotting, solution
Synonym(s):
hyb buffer, hyb solution, hybridization solution, northern hybridization buffer, southern hybridization buffer
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About This Item
NACRES:
NA.25
UNSPSC Code:
12161700
General description
Sigma′s PerfectHyb Plus is a ready-to-use hybridization buffer for Northern and Southern blot protocols. Use of PerfectHyb Plus can decrease hybridization time to as little as two hours.
Application
Suitable for use in hybridization buffers used in Northern blot, Southern blot, colony/plaque blots, and dot blots.
Features and Benefits
- Fast and easy-to-use
- Up to 20x more sensitive than conventional buffers
- Doesn′t require blocking steps
- Compatible with neutral and positively-charged membranes
- Compatible with chemiluminescent, colorimetric , and DIG detection assays
- Compatible with radioactive and non-radioactive probes
- Compatible with single or double-stranded DNA, RNA, and oligonucleotide probes
Other Notes
A small amount of precipitate that has formed in the solution can be dissolved at 30-35°C with occasional gentle inversion.
Legal Information
PerfectHyb is a trademark of Sigma-Aldrich Co. LLC
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Instructions
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Shinpei Kawaoka et al.
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TheScientificWorldJournal, 11, 102-117 (2011-01-11)
This protocol describes how to perform northern blot analyses to detect microRNAs and their precursors with single-nucleotide resolution, which is crucial for analyzing individual length variants and for evaluating relative quantities of unique microRNAs in cells. Northern blot analysis consists