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648310-M

Tris Base, Molecular Biology Grade

Name: Tris Base, Molecular Biology Grade
Brand: MM

≥99% (perchloric acid titration), solid, pH 7.0-9.0, solid, Molecular Biology Grade

Synonym(s):

Tris(hydroxymethyl)aminomethane, tris(Hydroxymethyl)aminomethane, 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Trometamol

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About This Item

Linear Formula:

NH₂C(CH₂OH)₃

CAS Number:

77-86-1

Molecular Weight:

121.14

UNSPSC Code:

12161705

NACRES:

NA.25

MDL number:

MFCD00004679

Beilstein/REAXYS Number:

741883

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Properties

Product Name

Tris Base, Molecular Biology Grade, TRIS base is useful in the pH range of 7.0-9.0. Has a pKa of 8.1 at 25°C.

grade

Molecular Biology

Quality Segment

100

description

Merck USA index - 14, 9772

assay

≥99% (perchloric acid titration)

form

solid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze

color

white

useful pH range

7-9

pKa (25 °C)

8.1

bp

219-220 °C/10 mmHg (lit.)

mp

167-172 °C (lit.)

solubility

water: 500 mg/mL

cation traces

heavy metals: ≤0.0005%

absorption

≤0.02 at 280 nm, ≤0.03 at 260 nm

foreign activity

RNases, DNases, proteases, none detected

storage temp.

10-30°C

SMILES string

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

InChI key

LENZDBCJOHFCAS-UHFFFAOYSA-N

Description

General description

Useful in the pH range of 7.0-9.0. Has a pK_a of 8.1 at 25°C.

Widely used as a biological buffer (absorbance: ≤0.03 at 260 nm; ≤0.02 at 280 nm). Useful in the pH range of 7.0-9.0. Has a pK_a of 8.1 at 25°C.

Application

A Fast and Automated Melanin-bleaching Method for Histopathologic Evaluation of Pigmented Melanoma Tissues.: This study presents an automated melanin-bleaching technique using a Tris-based buffer to enhance histopathologic evaluation of pigmented melanoma tissues, aiding in more accurate diagnoses (Ugolini et al., 2022).

Improvement of plant protein solubilization and 2-DE gel resolution through optimization of the concentration of Tris in the solubilization buffer.: Highlights the optimization of Tris concentration in solubilization buffers to enhance protein solubilization and gel electrophoresis resolution, crucial for biochemical studies (Cho et al., 2010).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c