F3165

ANTI-FLAG^® M2 antibody, Mouse monoclonal

Name: ANTI-FLAG<SUP>®</SUP> M2 antibody, Mouse monoclonal
Brand: SIGMA

clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

Synonym(s):

Anti-ddddk, Anti-dykddddk, Monoclonal ANTI-FLAG^® M2

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About This Item

NACRES:

NA.32

UNSPSC Code:

12352203

Product Name

ANTI-FLAG^® M2 antibody, Mouse monoclonal, clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin (Purified IgG1 subclass)

antibody product type

primary antibodies

clone

M2, monoclonal

form

buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

purified by

using Protein A

species reactivity

all

concentration

3.8-4.2 mg/mL

technique(s)

western blot: 10 μg/mL (Protein A)

isotype

IgG1

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

Related Categories

FLAG Purification

Protein Purification Chromatography

Protein Sample Prep

Immunogen

FLAG; peptide sequence DYKDDDDK

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Preparation Note

Dilute the antibody solution from 0.5-10 ug/mL in Tris Buffered Saline, pH 8.0, with 3% nonfat milk

Store the undiluted antibody at –20 °C in working aliquots. Repeated freezing and thawing is not recommended.
Note: Overtime, small amounts of purified antibodies can precipitate from solution due to intermolecular hydrophobic interactions. If a precipitate is observed in this product, briefly centrifuge the vial to pellet the precipitate. Withdraw the desired volume of antibody solution from the clear supernatant for use. This should not alter the performance of the purified antibody in Western blot or immunoprecipitation applications.

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Application

Monoclonal ANTI-FLAG^® M2 antibody has been used in:

immunoblotting
immunoprecipitation
immunocytochemistry
immunofluorescence
ELISA
EIA
chromatin immunoprecipitation
electron microscopy
flow cytometry
supershift assays

Browse additional application references in our FLAG^® Literature portal.

General description

Anti-Flag M2 antibody is used for the detection of Flag fusion proteins. This monoclonal antibody recognizes the FLAG sequence at the N-terminus, Met N-terminus, and C-terminus. The antibody is also able to recognize FLAG at an internal site. M2, unlike M1 antibody is not Calcium dependent.

F3165 is affinity purified using Protein A resin, so it contains not only the anti-FLAG M2 antibody but also small amounts of native mouse IgG, increasing its sensitivity in most applications.

Method of purification - Protein A

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Alternate subunit assembly diversifies the function of a bacterial toxin.

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Nature communications, 10(1), 3684-3684 (2019-08-17)

Bacterial toxins with an AB5 architecture consist of an active (A) subunit inserted into a ring-like platform comprised of five delivery (B) subunits. Salmonella Typhi, the cause of typhoid fever, produces an unusual A2B5 toxin known as typhoid toxin. Here

Molecular genetic analysis of VRK1 in mammary epithelial cells: depletion slows proliferation in vitro and tumor growth and metastasis in vivo.

T P Molitor et al.

Oncogenesis, 2, e48-e48 (2013-06-05)

The vaccinia-related kinases (VRKs) comprise a branch of the casein kinase family. VRK1, a ser/thr kinase with a nuclear localization, is the most well-studied paralog and has been described as a proproliferative protein. In lower eukaryotes, a loss of VRK1

Mutation of SIMPLE in Charcot-Marie-Tooth 1C alters production of exosomes.

Hong Zhu et al.

Molecular biology of the cell, 24(11), 1619-1637 (2013-04-12)

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AAV-ie enables safe and efficient gene transfer to inner ear cells.

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Nature communications, 10(1), 3733-3733 (2019-08-21)

Hearing loss is the most common sensory disorder. While gene therapy has emerged as a promising treatment of inherited diseases like hearing loss, it is dependent on the identification of gene delivery vectors. Adeno-associated virus (AAV) vector-mediated gene therapy has

Defects of mitochondrial RNA turnover lead to the accumulation of double-stranded RNA in vivo.

Aleksandra Pajak et al.

PLoS genetics, 15(7), e1008240-e1008240 (2019-08-01)

The RNA helicase SUV3 and the polynucleotide phosphorylase PNPase are involved in the degradation of mitochondrial mRNAs but their roles in vivo are not fully understood. Additionally, upstream processes, such as transcript maturation, have been linked to some of these

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ANTI-FLAG® M2 antibody, Mouse monoclonal