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F7425

ANTI-FLAG^® antibody produced in rabbit

Name: ANTI-FLAG<SUP>®</SUP> antibody produced in rabbit
Brand: SIGMA

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-ddddk, Anti-dykddddk

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.32

MDL number:

MFCD02262912

Conjugate:

unconjugated

Clone:

polyclonal

Application:

DB, FLAG purification, IF, IP, WB CL

Citations:

3023

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Properties

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

all

concentration

~0.8 mg/mL

technique(s)

FLAG purification: suitable, dot blot: 1-2.5 μg/mL, immunoprecipitation (IP): 4-8 μg using amino terminal FLAG-BAP fusion protein from E. coli crude lysate, indirect immunofluorescence: 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK, western blot (chemiluminescent): 1-2.5 μg/mL using an E. coli periplasmic extract expressing an N-terminal FLAG fusion protein

isotype

IgG

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

Description

General description

The rabbit Anti-FLAG polyclonal affinity antibody ANTI-FLAG recognizes the FLAG epitope located on FLAG fusion proteins. This antibody reacts with N-terminal, N-terminal-Met, and C-terminal FLAG fusion proteins.

The FLAG epitope, is an eight amino acid protein with an enterokinase-cleavage site.

Immunogen

FLAG; peptide sequence DYKDDDDK

Application

The antibody recognizes the FLAG epitope located on FLAG-tagged fusion proteins at the N-terminus or C-terminus, applying dot blot, immunoblotting, immunoprecipitation and immunocytochemistry assays. Learn more product details in our FLAG^® applicationportal."

Biochem/physiol Actions

FLAG epitope plays a crucial role in immunoaffinity purification of fusion proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Preparation Note

Purified by affinity chromatography on a column bearing the immunizing peptide.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

Flash Point (°F)

Not applicable

Flash Point (°C)