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G5885

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides

lyophilized powder, >= 550 units/mg protein (biuret)

Synonym(s):

G-6-P-DH

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About This Item

CAS Number:
9001-40-5
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-602-6
MDL number:
MFCD00081656
EC Number:
1.1.1.49 (BRENDA, IUBMB)

Product Name

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides, lyophilized powder, >= 550 units/mg protein (biuret)

biological source

bacterial (Leuconostoc mesenteroides)

type

Type XXIV

form

lyophilized powder

specific activity

>= 550 units/mg protein (biuret)

mol wt

128 kDa

composition

Protein, 15-40% biuret

application(s)

agriculture

foreign activity

6-Phosphogluconic dehydrogenase, hexokinase, NADH oxidase and NADPH oxidase ≤0.005%
PGI ≤0.01%

storage temp.

2-8°C

Quality Level

300

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Application

Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides has been used along with hexokinase in the determination of glucose from mice liver samples.

Biochem/physiol Actions

Glucose-6-Phophate Dehydrogenase (G-6-PDH) can utilize either nicotinamide adenine dinucleotide phosphate (NADP+) or NAD+ as coenzyme making it crucial for bacterial metabolism.
Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

General description

Glucose-6-Phophate Dehydrogenase (G-6-PDH) comprises His-Asp catalytic dyad and exists as a homodimer. Structurally, G-6-PDH encompasses a Rossmann dinucleotide binding fold in the coenzyme binding domain region. It also harbors a large β + α domain and has a unique aspartate residue at position 374.

Other Notes

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.

Physical form

Lyophilized powder containing Ficoll and Tris buffer salts

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000476088
0000476088
0000402989
0000402987
0000402990

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

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    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. In which solvent can Product No. G5885, Glucose- 6-phosphate Dehydrogenase from Leuconostoc mesenteroides, be dissolved?

    The product can be dissolved at 1 mg/ml in 5 mM glycine buffer , pH 8, (with or without 0.1% BSA)or in deionized water at 1 mg/ml (buffer is preferable).

  6. Can solutions of Product No. G5885, Glucose-6-phosphate Dehydrogenase, recombinant from Leuconostoc  mesenteroides, be stored?

    Solutions in buffer may be aliquotted and stored frozen at -20°C for about 1-2 months.

  7. What are the differences between Product No. G8529, Glucose-6-phosphate Dehydrogenase and Product No. G5885, Glucose-6-phosphate Dehydrogenase?

    Product No. G8529 is a recombinant protein from Leuconostoc mesenteroides, expressed in Escherichia coli.  Product No. G5885 is a non-recombinant protein from Leuconostoc mesenteroides.

  8. What is the molecular weight of Product No. G5885, Glucose-6-phosphate Dehydrogenase?

    Product No. G8529 is a recombinant protein from Leuconostoc  mesenteroides, expressed in Escherichia coli.  Product No. G5885 is a non-recombinant protein from Leuconostoc mesenteroides.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

V Vought et al.
Biochemistry, 39(49), 15012-15021 (2000-12-07)
The roles of particular amino acids in substrate and coenzyme binding and catalysis of glucose-6-phosphate dehydrogenase of Leuconostoc mesenteroides have been investigated by site-directed mutagenesis, kinetic analysis, and determination of binding constants. The enzyme from this species has functional dual
M S Cosgrove et al.
Biochemistry, 39(49), 15002-15011 (2000-12-07)
The role of Asp-177 in the His-Asp catalytic dyad of glucose 6-phosphate dehydrogenase from Leuconostoc mesenteroides has been investigated by a structural and functional characterization of the D177N mutant enzyme. Its three-dimensional structure has been determined by X-ray cryocrystallography in
J Pozueta-Romero et al.
FEBS letters, 291(2), 233-237 (1991-10-21)
The standardized enzyme coupling method for assaying sucrose synthase activities in the direction of sucrose cleavage was reexamined using enzyme preparations from cultured cells of sycamore (Acer pseudoplatanus L.) and spinach leaves (Spinacea oleracea). Both ATP and Tris, commonly utilized
A simple ultramicro method for determination of pyridine nucleotides in tissues.
J S Nisselbaum et al.
Analytical biochemistry, 27(2), 212-217 (1969-02-01)
Sofia Garcia et al.
Human molecular genetics, 31(5), 692-704 (2021-09-25)
We analyzed early brain metabolic adaptations in response to mitochondrial dysfunction in a mouse model of mitochondrial encephalopathy with complex IV deficiency [neuron-specific COX10 knockout (KO)]. In this mouse model, the onset of the mitochondrial defect did not coincide with
View All Related Papers

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Product Information Sheet - G5885

Product Information Sheet

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