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Merck

SML1661

Bafilomycin A1

from Streptomyces griseus, ≥90% (HPLC), DMSO solution, V-ATPase inhibitor

Synonym(s):

BafA1

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About This Item

Empirical Formula (Hill Notation):
C35H58O9
CAS Number:
88899-55-2
Molecular Weight:
622.83
UNSPSC Code:
12352200
NACRES:
NA.77

Product Name

Bafilomycin A1 Ready Made Solution, 0.16 mM in DMSO, from Streptomyces griseus

SMILES string

O1[C@@H]([C@H]([C@@H](C[C@]1(O)[C@H]([C@H](O)[C@@H]([C@H]2OC(=O)\C(=C\C(=C\[C@H]([C@H]([C@H](C\C(=C\C=C\[C@@H]2OC)\C)C)O)C)\C)\OC)C)C)O)C)C(C)C

InChI

1S/C35H58O9/c1-19(2)32-24(7)27(36)18-35(40,44-32)26(9)31(38)25(8)33-28(41-10)14-12-13-20(3)15-22(5)30(37)23(6)16-21(4)17-29(42-11)34(39)43-33/h12-14,16-17,19,22-28,30-33,36-38,40H,15,18H2,1-11H3/b14-12+,20-13+,21-16+,29-17-/t22-,23+,24-,25-,26-,27+,28-,30-,31+,32+,33+,35+/m0/s1

InChI key

XDHNQDDQEHDUTM-JQWOJBOSSA-N

biological source

Streptomyces griseus

form

DMSO solution

concentration

0.16 mM in DMSO

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

Related Categories

Other Notes

Bafilomycin A1 solution is provided in concentration of 0.16 mM. Typical concentrations for use in cell culture are 50-100 nM.

Application

Bafilomycin A1 has been used:
  • as an endosome acidification inhibitor to study the importance of endosome acidification in the extracellular vesicle uptake and cytosolic release of stably expressing NanoLuc luciferase-tagged Hsp70 (NLuc-Hsp70) in HeLa cells
  • as a vacuolar-type H+-ATPase (V-ATPase) inhibitor to study its effects on autophagic turnover of light chain 3 β (LC3-II) in mice
  • as an autophagy inhibitor to study its effects on primary rat liver sinusoidal endothelial cells (LSECs) defenestration

Biochem/physiol Actions

Bafilomycin A1 inhibits autophagy. It may exhibit anti-tumorigenic, anti-parasitic, and anti-neurodegenerative effects. Bafilomycin A1, found in lysosomes and endosomes prevents the acidification of these cell organelles. It also participates in blocking autophagosome-lysosome fusion and autolysosome acidification, steps necessary for maintaining the autophagic flux and cellular homeostasis.
Bafilomycin A1 is a macrolide antibiotic.
Bafilomycin A1 is a macrolide antibiotic. Bafilomycin A1 acts as a potent and selective inhibitor of vacuolar-type H+-ATPase.

General description

Bafilomycin A1, a macrolide antibiotic, belongs to the pleomacrolides family. It acts as a potent and selective inhibitor of vacuolar-type H+-ATPase. BafA1 can inhibit the viability of MG63 osteosarcoma cells. It can also stimulate mitochondrial dysfunction. BafA1 inhibits the proliferation of different types of cancer cells.

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

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Certificates of Analysis (COA)

Lot/Batch Number
0000409695
0000409695
0000328284
0000328284
0000284577

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Bafilomycin A1 induces caspase-independent cell death in hepatocellular carcinoma cells via targeting of autophagy and MAPK pathways
Yan Y, et al.
Scientific Reports (2016)
Bafilomycin A1 inhibits autophagy and induces apoptosis in MG63 osteosarcoma cells
Xie Z, et al.
Molecular medicine reports (2014)
Molecular basis of V-ATPase inhibition by bafilomycin A1
Wang R, et al.
Nature Communications (2021)
Célia Fourrier et al.
Biochemical and biophysical research communications, 534, 107-113 (2020-12-15)
Measurement of autophagic flux in vivo is critical to understand how autophagy can be used to combat disease. Neurodegenerative diseases have a special relationship with autophagy, which makes measurement of autophagy in the brain a significant research priority. Currently, measurement of
Xiaoying Luo et al.
Cell death & disease, 9(5), 576-576 (2018-05-16)
Autophagy, interacting with actin cytoskeleton and the NO-dependent pathway, may affect the phenotype and function of endothelial cells. Moreover, caveolin-1 (Cav-1), as a structure protein in liver sinusoidal endothelial cells (LSECs), is closely related to autophagy. Hence, we aim to
View All Related Papers

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