R4526
Reference Dye for Quantitative PCR
100 ×, solution
Synonym(s):
Reference dye for qPCR, ROX
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About This Item
NACRES:
NA.55
UNSPSC Code:
41106300
form
solution
Quality Level
usage
sufficient for ≥600 reactions
feature
PCR Additives
concentration
100 ×
technique(s)
PCR: suitable
color
red
input
crude DNA
solubility
water: soluble
storage temp.
2-8°C
General description
Sigma′s Reference Dye for quantitative polymerase chain reaction (qPCR) is a proprietary dye for use with real-time PCR. It is used for the normalization of reaction data when using SYBR Green, molecular probes, or dual-labeled probe chemistries for real-time detection. The Reference Dye is supplied as a 100× solution with a maximum excitation and emission of 586 nm and 605 nm, respectively. Instrument settings for ROX reference dye are satisfactory for the measurement of Reference Dye for qPCR.
Application
Reference Dye for Quantitative PCR has been used:
- in the preparation of mastermix for real time-quantitative polymerase chain reaction (RT-qPCR)
- as a component of the reaction mixture for detection of Clostridium difficile by quantitative polymerase chain reaction (qPCR)
- for analyzing the degree of cellular DNA contamination by qPCR
Other Notes
Reference Dye for Quantitative PCR is forR&D use only. Not for drug, household, or other uses.
signalword
Warning
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
ppe
Eyeshields, Gloves
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