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Merck

162892

Purpald®

≥99%

Synonym(s):

4-Amino-3-hydrazino-5-mercapto-1,2,4-triazole, 4-Amino-5-hydrazino-1,2,4-triazole-3-thiol

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About This Item

Empirical Formula (Hill Notation):
C2H6N6S
CAS Number:
1750-12-5
Molecular Weight:
146.17
UNSPSC Code:
12352100
NACRES:
NA.22
PubChem Substance ID:
24850011
EC Number:
217-135-8
Beilstein/REAXYS Number:
134867
MDL number:
MFCD00003098
Assay:
≥99%
Form:
powder

Key Documents

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Quality Level

200

assay

≥99%

form

powder

mp

228-230 °C (dec.) (lit.)

solubility

0.5 M NaOH: soluble 50 mg/mL, clear to slightly hazy (yellow to yellow brown or orange solution)

functional group

hydrazine

SMILES string

NNc1nnc(S)n1N

InChI

1S/C2H6N6S/c3-5-1-6-7-2(9)8(1)4/h3-4H2,(H,5,6)(H,7,9)

InChI key

RDIMQHBOTMWMJA-UHFFFAOYSA-N

Application

  • Purpald® was used in purpald assay for determination of bacterial polysaccharides containing substituted or unsubstituted glycol in residues such as glycerol, ribitol, arabinitol, furanosyl galactose and sialic acid.
  • It was used as chromogen during the determination of catalase activity in small tissue samples.
  • It was used as reagent in determination of formaldehyde.
  • It was used for derivatization of neutral monosaccharides during HPLC.

Legal Information

Purpald is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Flame
GHS02

signalword

Danger

hcodes

H228

Hazard Classifications

Flam. Sol. 1

Storage Class

4.1A - Other explosive hazardous materials

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SDBB7673
SDBB2381
STBL2340
STBK7566
STBJ8487

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Journal of Liquid Chromatography, 16, 1105-1105 (1993)
Ashraf Gholizadeh
Physiology and molecular biology of plants : an international journal of functional plant biology, 26(4), 829-839 (2020-04-08)
DUF538 (domain of unknown function 538) proteins are known as a group of putative hypothetical proteins in a wide range of plant species. They have been identified from some plants challenged with various environmental stresses. However, a little is known
J S Beckman et al.
Proceedings of the National Academy of Sciences of the United States of America, 87(4), 1620-1624 (1990-02-01)
Superoxide dismutase reduces injury in many disease processes, implicating superoxide anion radical (O2-.) as a toxic species in vivo. A critical target of superoxide may be nitric oxide (NO.) produced by endothelium, macrophages, neutrophils, and brain synaptosomes. Superoxide and NO.
Gordon E Anthon et al.
Journal of agricultural and food chemistry, 52(12), 3749-3753 (2004-06-10)
Three colorimetric reagents for the determination of formaldehyde, the Nash reagent (ammonia plus acetylacetone), Purpald (4-amino-3-hydrazino-5-mercapto-1,2,4-triazole), and N-methylbenzothiazolinone-2-hydrazone (MBTH), were compared for the determination of methanol when used in conjunction with alcohol oxidase. The combination of alcohol oxidase plus the
C H Lee et al.
Analytical biochemistry, 267(1), 161-168 (1999-01-27)
We have adapted the purpald assay (M. S. Quesenberry and Y. C. Lee, Anal. Biochem. 234, 50-55, 1996) to quantify lipopolysaccharide (LPS) content in solution in 96-well microtiter plates at room temperature. This method employs the oxidation of unsubstituted terminal
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