04-147

Anti-ASC Antibody, clone 2EI-7

Name: Anti-ASC Antibody, clone 2EI-7
Brand: MM

clone 2EI-7, from mouse

Synonym(s):

Caspase recruitment domain-containing protein 5, PYD and CARD domain containing, PYD and CARD domain-containing protein, Target of methylation-induced silencing 1, apoptosis-associated speck-like protein containing a CARD, caspase recruitment domain prot

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Product Name

Anti-ASC Antibody, clone 2EI-7, clone 2EI-7, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

2EI-7, monoclonal

species reactivity

mouse

species reactivity (predicted by homology)

human (based on 100% sequence homology)

technique(s)

flow cytometry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NM_145182

UniProt accession no.

Q9ULZ3

shipped in

wet ice

target post-translational modification

unmodified

Analysis Note

Control
Mouse spleen tissue lysate

Evaluated by Western Blot on mouse spleen tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected ASC in mouse spleen tissue lysate.

Application

Detect ASC using this Anti-ASC Antibody, clone 2EI-7 validated for use in WB, FC.

Research Category
Inflammation & Immunology

Research Sub Category
Apoptosis - Additional

Biochem/physiol Actions

This antibody recognizes ASC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK, Bcl10, Apaf-1, ARC, caspase-2 and caspase-9. A novel CARD domain containing protein has been identified in human and mouse and designated ASC and TMS1. Irregular expression of ASC/TMS1 induced apoptosis through activation of caspase-9 and inhibited the survival of human breast cancer cells. Overexpression of ASC/TMS1 induced DNA fragmentation. ASC/TMS1 is expressed in a variety of human and mouse tissues.

~22 kDa

Immunogen

Epitope: Unknown

Full length recombinant human ASC.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The PYRIN domain-only protein POP3 inhibits ALR inflammasomes and regulates responses to infection with DNA viruses.

Sonal Khare et al.

Nature immunology, 15(4), 343-353 (2014-02-18)

The innate immune system responds to infection and tissue damage by activating cytosolic sensory complexes called 'inflammasomes'. Cytosolic DNA is sensed by AIM2-like receptors (ALRs) during bacterial and viral infections and in autoimmune diseases. Subsequently, recruitment of the inflammasome adaptor

NLRP3 inflammasome activation contributes to long-term behavioral alterations in mice injected with lipopolysaccharide.

Wei Zhu et al.

Neuroscience, 343, 77-84 (2016-12-08)

Lipopolysaccharide (LPS) might affect the central nervous system by causing neuroinflammation, which subsequently leads to brain damage and dysfunction. In this study, we evaluated the role of nod-like receptor pyrin domain-containing protein 3 (NLRP3) inflammasome activation in long-term behavioral alterations

Chromatin dynamics during DSB repair.

Martin Falk,Emilie Lukasova,Barbora Gabrielova,Vladan Ondrej,Stanislav Kozubek

Biochimica et Biophysica Acta null

CLICs-dependent chloride efflux is an essential and proximal upstream event for NLRP3 inflammasome activation.

Tiantian Tang et al.

Nature communications, 8(1), 202-202 (2017-08-06)

The NLRP3 inflammasome can sense different pathogens or danger signals, and has been reported to be involved in the development of many human diseases. Potassium efflux and mitochondrial damage are both reported to mediate NLRP3 inflammasome activation, but the underlying

Carbon monoxide negatively regulates NLRP3 inflammasome activation in macrophages.

Sung-Soo Jung et al.

American journal of physiology. Lung cellular and molecular physiology, 308(10), L1058-L1067 (2015-03-15)

Inflammasomes are cytosolic protein complexes that promote the cleavage of caspase-1, which leads to the maturation and secretion of proinflammatory cytokines, including interleukin-1β (IL-1β) and IL-18. Among the known inflammasomes, the nucleotide-binding domain, leucine-rich-containing family, pyrin domain-containing-3 (NLRP3)-dependent inflammasome is

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Global Trade Item Number

SKU	GTIN
04-147	04053252586224

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