Anti-EAF1 Antibody, clone 1E6

ELL-associated factor 1 (UniProt: Q96JC9; also known as EAF1) is encoded by the EAF1 gene (Gene ID: 85403) in human. EAF1, a member of the EAF family, is a component of the super elongation complex that is composed of EAF1, EAF2, CDK9, MLLT3/AF9, AFF, the P-TEFb complex and ELL. It acts as a transcriptional transactivator of ELL and ELL2 elongation activities. In its C-terminal region resides a sequence (aa 182-262) that is shown to be essential for its transactivation activity. The carboxy-terminal of ELL contains a sequence (aa 508-621) that is shown to be essential for interaction with EAF1. Both EAF1 and EAF2 are shown to play important roles in tumor suppression and embryogenesis. They are also reported to inhibit Wnt/β-catenin signaling, thereby modulating mesodermal and neural patterning in the embryo and their ectopic expression in embryos and cultured cells can block β-catenin reporter activity. They interact with the β-catenin center region containing 12 Armadillo repeats and the C-terminus region. EAF1 is strongly expressed in many tissues, including heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, prostate, testis, small intestine, and colon. Two isoforms of EAF1 have been described that are produced by alternative splicing. (Ref.: Liu, JX., et al. (2013). Development. 140(5); 1067-1078; Simone, F., et al. (2003). Blood. 101(6); 2355-2362; Simone, F., et al. (2001); Blood 98(1); 201-209).

His-tagged full-length recombinant human ELL-associated factor 1 (EAF1).

Quality Control Testing

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected EAF1 in HeLa cells.

Tested Applications

Immunocytochemistry Analysis: A representative lot detected EAF1 in Immunocytochemistry applications (Simone, F., et al. (2001). Blood.;98(1):201-9).

Immunoprecipitation Analysis: A representative lot immunoprecipitated EAF1 in Immunoprecipitation applications (Simone, F., et al. (2001). Blood.;98(1):201-9; Simone, F., et al. (2003). Blood.;101(6):2355-62).

Western Blotting Analysis: A representative lot detected EAF1 in Western Blotting applications (Simone, F., et al. (2001). Blood.;98(1):201-9).

Northern Blotting: A representative lot detected EAF1 in Northern Blotting applications (Simone, F., et al. (2001). Blood.;98(1):201-9).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-EAF1, clone 1E6, Cat. No. MABE1989, is a mouse monoclonal antibody that detects ELL-associated factor 1 and is tested for use in Immunocytochemistry, Immunoprecipitation, Northern Blotting, and Western Blotting.

Clone 1F6 is a mouse monoclonal antibody that detects human ELL-associated factor 1 (EAF1).

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.