MABE451
Anti-p31comet Antibody, clone E29.19.14
clone E29.19.14, from mouse
Synonym(s):
MAD2L1-binding protein, Caught by MAD2 protein
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
E29.19.14, monoclonal
Application:
IP, WB
Citations:
3
Quality Level
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
E29.19.14, monoclonal
species reactivity
human
technique(s)
immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... MAD2L1BP(9587)
General description
35 kDa observed
p31comet is also called MAD2L1-binding protein (MAD2L1BP) and Caught by MAD2 protein (CMT2). p31comet silences the spindle checkpoint and allows mitosis to proceed through anaphase by binding MAD2L1 after it has become dissociated from the MAD2L1-CDC20 complex. p31comet is expressed throughout the cell cycle, with levels increasing and then remaining constant until late mitosis after which they drop.
Immunogen
Recombinant protein corresponding to human p31comet.
Application
Detect p31comet using this mouse monoclonal antibody, Anti-p31comet Antibody, clone E29.19.14 validated for use in western blotting & IP.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
Western Blotting Analysis: A representative lot from an independent laboratory detected p31comet in HeLa cells arrested in prometaphase (Mansfeld, J., et al. (2011). Nat Cell Biol. 13(10):1234-1243.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory detected p31comet in HeLa cell lysates, which were untreated (asynchronous), arrested in nocodazole for 16 hr, or released in fresh medium for 2 hr (Varetti, G., et al. (2011). 44(5):710-720.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory detected p31comet in HeLa cell lysates, which were untreated (asynchronous), arrested in nocodazole for 16 hr, or released in fresh medium for 2 hr (Varetti, G., et al. (2011). 44(5):710-720.).
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected p31comet in 10 µg of HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected p31comet in 10 µg of HeLa cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Prabha Sarangi et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(43), 26795-26803 (2020-10-15)
The repair of DNA double strand breaks (DSBs) that arise from external mutagenic agents and routine cellular processes is essential for life. DSBs are repaired by two major pathways, homologous recombination (HR) and classical nonhomologous end joining (C-NHEJ). DSB repair
You Heng Chuah et al.
Cell death and differentiation, 30(8), 1973-1987 (2023-07-20)
MAD2 is a spindle assembly checkpoint protein that participates in the formation of mitotic checkpoint complex, which blocks mitotic progression. RNF8, an established DNA damage response protein, has been implicated in mitotic checkpoint regulation but its exact role remains poorly
Antonio Cuevas-Navarro et al.
Current biology : CB, 31(17), 3915-3924 (2021-07-09)
The spindle assembly checkpoint (SAC) functions as a sensor of unattached kinetochores that delays mitotic progression into anaphase until proper chromosome segregation is guaranteed.1,2 Disruptions to this safety mechanism lead to genomic instability and aneuploidy, which serve as the genetic
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MABE451 | 04053252923906 |