B5144
Bromelain from pineapple stem
≥4 units/mg protein, (chromatography purified)
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-387-5
MDL number:
Specific activity:
(chromatography purified), ≥4 units/mg protein
form
lyophilized powder
specific activity
(chromatography purified), ≥4 units/mg protein
composition
Protein, 30-50% biuret
solubility
H2O: soluble 1.0 mg/mL, clear, colorless
storage temp.
−20°C
Quality Level
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Application
Bromelain from pineapple stem has been used as a positive control in sodium dodecyl sulfate (SDS) / polyacrylamide gel electrophoresis (PAGE) zymograms. It has also been used as a Azocasein substrate for the determination of proteolytic activity.
Bromelain may be used to inhibit the biosysnthesis of proinflammatory prostaglandins. It may be used to reduce clotting efficiency. Bromelain, from pineapple stem, has been used to make enzymatic hydrolysates of honeybee-collected pollen .
Biochem/physiol Actions
Bromelain is a cysteine endopeptidase with broad specificity for cleavage of proteins. Bromelain may be from a stem or piece of fruit. Product P3169 (pGlu-Phe-Leu-p-nitroanilide) may be used as a chromogenic substrate for the assay of bromelain.
Physical form
Lyophilized powder containing mannitol and potassium phosphate buffer salts
Preparation Note
Chromatographically purified
Chromatographically purified. Dissolves in water at 1 mg/mL concentration to form a clear, colorless solution.
Other Notes
One unit will release 1.0 μmole of p-nitrophenol from N-α-Z-L-lysine p-nitrophenyl ester per min at pH 4.6 at 25 °C.
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
13 - Non Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Azocasein substrate for determination of proteolytic activity: reexamining a traditional method using bromelain samples
Coelho DF, et al.
BioMed Research International (2016)
Larisa V Kordyukova et al.
Membranes, 11(10) (2021-10-23)
Influenza A virus envelope contains lipid molecules of the host cell and three integral viral proteins: major hemagglutinin, neuraminidase, and minor M2 protein. Membrane-associated M1 matrix protein is thought to interact with the lipid bilayer and cytoplasmic domains of integral
Acidic cysteine protease inhibitors from pineapple stem.
R L Heinrikson et al.
Methods in enzymology, 45, 740-751 (1976-01-01)
Ashley G Yow et al.
Scientific reports, 13(1), 11605-11605 (2023-07-19)
Papain (aka C1A) family proteases, including bromelain enzymes, are widespread across the plant kingdom and play critical regulatory functions in protein turnover during development. The proteolytic activity exhibited by papain family proteases has led to their increased usage for a
Basir Ahmad et al.
Biopolymers, 81(5), 350-359 (2005-12-14)
The effect of low, medium, and high molecular weight poly(ethylene glycol) (e.g., PEG-400, -6000, and -20,000) on the structure of the acid unfolded state of unmodified stem bromelain (SB) obtained at pH 2.0 has been studied by various spectroscopic methods.
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