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C0543

Mouse Collagen Type IV

Name: Mouse Collagen Type IV
Brand: SIGMA

from Engelbreth-Holm-Swarm murine sarcoma basement membrane, lyophilized powder, suitable for cell culture, used for 3D hydrogel formation

Synonym(s):

Type IV collagen, EHS-collagen

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About This Item

CAS Number:

9007-34-5

UNSPSC Code:

12352202

NACRES:

NA.75

EC Number:

232-697-4

MDL number:

MFCD00130825

Biological source:

mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)

Form:

lyophilized powder

Technique(s):

cell culture | mammalian: suitable

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Properties

Product Name

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane, Type IV (Miller), lyophilized powder, BioReagent, suitable for cell culture

biological source

mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)

Quality Level

200

type

Type IV (Miller)

product line

BioReagent

form

lyophilized powder

packaging

glass bottle of 1 vial

technique(s)

cell culture | mammalian: suitable

surface coverage

6‑10 μg/cm²

solubility

0.25% acetic acid: 0.5-2.0 mg/mL (Keep at 2-8 °C for several hours, occasionally swirling.)

UniProt accession no.

B1AVK5, P02463, P08122, Q63ZW6, Q9QZS0

shipped in

dry ice

storage temp.

−20°C

Gene Information

mouse ... Col4a1(12826), Col4a2(12827), Col4a3(12828), Col4a5(12830), Col4a6(94216)

Description

General description

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Collagen is classified into several structurally and genetically distinct types. Type IV collagen is a predominant structural protein of the basement membrane. It is notably characteristic of the Engelbreth-Holm-Swarm (EHS) mouse sarcoma. It is commercially available as matrigel and cultrex. Matrigel is made of type 4 collagen, laminin, heparan sulfate proteoglycan and entactin obtained from engelbreth-holm-swarm murine sarcoma. The target cells for attachment include: epithelial cells, endothelial cells, muscle cells, and nerve cells.

Application

Recommended for use as a cell culture substratum at 6-10 μg/cm².

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used:

in adhesion assays.
in hydrogel fabrication.
to coat cell culture plates for cell differentiation.
to treat round plastic coverslips for scanning electron microscopy (SEM) analysis in an interaction study between A. culbertsoni trophozoites and Schwann cells.

Biochem/physiol Actions

Collagen isolated from Engelbreth-Holm-Swarm murine sarcoma basement membrane helps in the development of 3D nodules. It is also used in 2D coatings.

Preparation Note

0.75 mg/vial

Chloroform treated.
Pepsin is not used in the preparation.

Other Notes

Native collagen

We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma-type designations with recognized collagen classification types.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000417010

0000209986

0000099955

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