E7885
Elastase from porcine pancreas
lyophilized powder, suitable for cell culture
Synonym(s):
Elastase from hog pancreas, Pancreatopeptidase E
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.75
EC Number:
254-453-6
MDL number:
Product Name
Elastase from porcine pancreas, lyophilized powder, suitable for cell culture
biological source
Porcine
form
lyophilized powder
specific activity
≥4 units/mg protein
mol wt
25.9 kDa
packaging
pkg of 1 μmol
technique(s)
cell culture | mammalian: suitable
pH
8
suitability
suitable for
foreign activity
Trypsin ≤50 BAEE units/mg protein
shipped in
dry ice
storage temp.
−20°C
Quality Level
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Application
Elastase from porcine pancreas is used for the following applications:
- Cell culture techniques
- ELISA techniques
- Used in assay for elastase inhibitory activity
Biochem/physiol Actions
Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.
Enzyme that degrades elastin, a matrix component of tissues involved in stretching processes. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester.
General description
Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin.
Other Notes
One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.
Packaging
Package size based on protein content
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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F Seibold et al.
Gut, 32(10), 1192-1197 (1991-10-01)
The presence of antibodies against pancreatic juice (PAB) in patients with Crohn's disease has recently been reported. In our study sera from 273 patients with inflammatory bowel disease (222 with Crohn's disease, 51 with ulcerative colitis) have been examined for
Yoshiyuki Okumura et al.
Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology, 48(1), 13-18 (2007-02-09)
The amino acid sequence of elastase inhibitor, AFLEI, isolated from Aspergillus flavus was determined by the Edman sequencing procedure of peptides derived from digests utilizing clostripain. A molecular weight of 7,525.8 was observed by TOF-MS. AFLEI contained 68 amino acid
Adrien Lupieri et al.
Cardiovascular research, 116(2), 438-449 (2019-05-21)
Defects in efficient endothelial healing have been associated with complication of atherosclerosis such as post-angioplasty neoatherosclerosis and plaque erosion leading to thrombus formation. However, current preventive strategies do not consider re-endothelialization in their design. Here, we investigate mechanisms linking immune
P Hamet et al.
The Journal of biological chemistry, 264(21), 12364-12369 (1989-07-25)
The induction of cyclic GMP formation in target tissues, i.e. vascular smooth muscle and endothelial cells, by atrial natriuretic factor is followed by its egression into plasma and urine. Since the extracellular appearance of this cyclic nucleotide is used as
Scott W Walsh et al.
Reproductive sciences (Thousand Oaks, Calif.), 27(1), 246-259 (2020-02-13)
Decidual macrophages secrete proteases that activate protease-activated receptor 1 (PAR-1). We hypothesized that activation of the inflammatory response by bacteria is amplified by proteases, initiating labor. In addition, we hypothesized that commensal bacteria trigger an inflammatory response by activating NF-κB
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