FLAAM
Adenosine 5′-triphosphate (ATP) assay mix
lyophilized powder
Synonym(s):
ATP assay kit, ATP detection kit
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About This Item
NACRES:
NA.84
eCl@ss:
32160414
UNSPSC Code:
41116133
form
lyophilized powder
technique(s)
PCR: suitable
storage temp.
−20°C
Quality Level
usage
vial sufficient for ≥50 assays
Related Categories
Application
Adenosine 5′-triphosphate (ATP) assay mix has also been used to measure the level of cellular ATP in ATP viability assay.
The ATP assay kit has been used to measure the level of cellular ATP present in isolated mitochondrial fractions from L. donovani promastigotes.
General description
The Adenosine 5’-triphosphate (ATP) Bioluminescent Assay Kit may be employed for the quantitative bioluminescent determination of adenosine 5’-triphosphate (ATP) in samples containing 2x10–12 to 8x10–5 moles/liter. ATP is consumed and light is emitted when luciferase catalyzes the oxidation of D-luciferin.
Other Notes
Contains luciferase, luciferin, MgSO4, EDTA, DTT, BSA, and Tricine buffer.
Preparation Note
Reconstitute with 5 ml of sterile water.
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Amit Roy et al.
Molecular pharmacology, 74(5), 1292-1307 (2008-08-16)
Mitochondria are the principal site for the generation of cellular ATP by oxidative phosphorylation. F0F1-ATP synthase, a complex V of the electron transport chain, is an important constituent of mitochondria-dependent signaling pathways involved in apoptosis. In the present study, we
Ann K Rosenthal et al.
Arthritis research & therapy, 15(5), R154-R154 (2013-11-30)
Extracellular ATP (eATP) is released by articular chondrocytes under physiological and pathological conditions. High eATP levels cause pathologic calcification, damage cartilage, and mediate pain. We recently showed that stable over-expression of the progressive ankylosis gene product, ANK, increased chondrocyte eATP
Nazlıhan Aztopal et al.
Chemico-biological interactions, 280, 51-58 (2017-12-12)
Cancer stem-like cells (CSCs) are a cell subpopulation that can reinitiate tumors, resist chemotherapy, give rise to metastases and lead to disease relapse because of an acquired resistance to apoptosis. Especially, epigenetic alterations play a crucial role in the regulation
Nobuyuki Nishikawa et al.
Neurourology and urodynamics, 39(6), 1644-1652 (2020-06-13)
Bladder wall stretch increases tissue tension and releases adenosine 5'-triphosphate (ATP) as part of a transduction process to sense bladder filling. Aging is associated with bladder fibrosis to produce a stiffer bladder wall: this may augment ATP release and contribute
Asako Kusumi et al.
Frontiers in microbiology, 2, 104-104 (2011-07-13)
To characterize sulfate-producing microorganisms from the deteriorated sandstones of Angkor monuments in Cambodia, strains of Mycobacterium spp. were isolated from most probable number-positive cultures. All five strains isolated were able to use both elemental sulfur (S(0)) for chemolithoautotrophic growth and
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