G5893
Gamborg′s B-5 Basal Medium with Minimal Organics
fine powder, suitable for plant cell culture
Synonym(s):
B-5 Basal Medium, Basal Medium, Minimal Organics Medium
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About This Item
NACRES:
NA.72
UNSPSC Code:
12352207
form
fine powder
technique(s)
cell culture | plant: suitable
application(s)
agriculture
storage temp.
2-8°C
Quality Level
General description
Gamborg′ B-5 medium may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg′s B-5 (B5) medium for in vitro culture of plant cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis. Gamborg′s B-5 medium has been used for the culture of Annona squamosa callus.
Gamborg′s B-5 basal medium is an efficient nutrient mixture for plants.
Application
Gamborg′s B-5 Basal Medium with Minimal Organics has also been used:
- as a component of liquid media for the tissue culture of Brassica rapa
- as a component of callus inducing medium for culturing Arabidopsis cardiolipin mutant seedlings
- as a component of solid GM medium for germination of Arabidopsis seeds
Preparation Note
Formulated to contain 3.2 grams of powder per liter of medium.
With the macro- and micronutrients, and vitamins as described by Gamborg, et al. (1968).
Media Formulation
Media Formulation
signalword
Warning
hcodes
Hazard Classifications
Ox. Sol. 3
Storage Class
5.1B - Oxidizing hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
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Experiments in Plant Tissue Culture (1985)
Jakob Petereit et al.
Frontiers in plant science, 8, 72-72 (2017-02-24)
In eukaryotes the presence of the dimeric phospholipid cardiolipin (CL) is limited to the mitochondrial membranes. It resides predominantly in the inner membrane where it interacts with components of the mitochondrial electron transfer chain. CL deficiency has previously been shown
RNAi-mediated down-regulation of DCL1 and AGO1 induces developmental changes in resynthesized Arabidopsis allotetraploids
Lackey E, et al.
The New phytologist, 186(1), 207-215 (2010)
Martin E Kolewe et al.
Plant cell reports, 29(5), 485-494 (2010-03-11)
Plant cells grow as aggregates in suspension culture, but little is known about the dynamics of aggregation, and no routine methodology exists to measure aggregate size. In this study, we evaluate several different methods to characterize aggregate size in Taxus
Metabolism of polyamines in transgenic cells of carrot expressing a mouse ornithine decarboxylase cDNA
Andersen S E, et al.
Plant Physiology, 116(1), 299-307 (1998)
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