G7294
Guanidine hydrochloride solution
8 M, pH 8.5, buffered aqueous solution
Synonym(s):
Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, GuHCl, Guanidine HCl, Guanidinium chloride, Guanidium chloride
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About This Item
NACRES:
NA.25
UNSPSC Code:
12352209
form
buffered aqueous solution
Quality Level
concentration
8 M
color
colorless
pH
8.5
application(s)
diagnostic assay manufacturing
Application
Guanidine hydrochloride solution has been used:
- as a denaturing agent during the preparation of HEK293T cells and in the resuspension buffer to prevent protein aggregation during the homogenization process in mouse tissue preparation.
- for the extraction of total RNA from plant samples.
- for the digestion of plasma and HDL proteins.
- for the denaturation of proteins during the conformational stability assay of misfolded prion proteins.
signalword
Warning
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
Storage Class
10 - Combustible liquids
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Related Content
The preparation of guanidine hydrochloride.
Nozaki, Yasuhiko
Methods in Enzymology, 26, 43-50 (1972)
Urea and guanidine hydrochloride denaturation of ribonuclease, lysozyme, alpha-chymotrypsin, and beta-lactoglobulin.
R F Greene et al.
The Journal of biological chemistry, 249(17), 5388-5393 (1974-09-10)
Extended conformations of polypeptides and proteins in urea and guanidine hydrochloride.
Tiffany, M. Lois, and Samuel Krimm
Biopolymers, 12, 575-587 (1973)