M5814
MKK6/SKK3, active human
≥80% (SDS-PAGE), recombinant, expressed in E. coli, Maltose binding protein tagged, buffered aqueous solution
Synonym(s):
MAP Kinase Kinase 6, MAP Kinase Kinase 6/SKK 3
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About This Item
Assay:
≥80% (SDS-PAGE)
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
Quality Level
assay
≥80% (SDS-PAGE)
form
buffered aqueous solution
mol wt
81 kDa
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... MAP2K6(5608)
Application
Active enzyme for study of stress-activated protein kinases.
May be used for activation of p38α followed by phosphorylation of MBP.
Biochem/physiol Actions
Active in the phosphorylation of p38/SAPK2a
Physical form
Solution in approx. 1 mg/ml in 50 mM Tris-HCl, pH 7.5, 0.1 mM EGTA, 0.1% 2-mercaptoethanol, 0.15 M NaCl, 0.03% Brij® L23, 270 mM sucrose, 0.2 mM PMSF, 1 mM benzamidine.
Other Notes
1 unit will activate 1 unit of p38/SAPK2 per minute. 1 unit of active p38/SAPK2 will incorporate 1 nanomole of phosphorus into myelin basic protein per minute.
Legal Information
Brij is a registered trademark of Croda International PLC
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Instructions
A Cuenda et al.
The EMBO journal, 16(2), 295-305 (1997-01-15)
Stress-activated protein kinase-3 (SAPK3), a recently described MAP kinase family member with a wide-spread tissue distribution, was transfected into several mammalian cell lines and shown to be activated in response to cellular stresses, interleukin-1 (IL-1) and tumour necrosis factor (TNF)