N1127
2-Nitrophenyl β-D-galactopyranoside
≥98% (enzymatic)
Synonym(s):
ONPG , o-Nitrophenyl β-D-galactopyranoside, ONPG
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About This Item
Empirical Formula (Hill Notation):
C12H15NO8
CAS Number:
Molecular Weight:
301.25
UNSPSC Code:
12352200
NACRES:
NA.52
PubChem Substance ID:
EC Number:
206-716-1
Beilstein/REAXYS Number:
92207
MDL number:
grade
Molecular Biology
Quality Level
assay
≥98% (enzymatic)
form
powder
storage temp.
−20°C
SMILES string
OC[C@H]1O[C@@H](Oc2ccccc2[N+]([O-])=O)[C@H](O)[C@@H](O)[C@H]1O
InChI
1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12-/m1/s1
InChI key
KUWPCJHYPSUOFW-YBXAARCKSA-N
General description
Chromogenic substrate for β-galactosidase
ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric substrate for β-galactosidase.
Application
2-Nitrophenyl β-D-galactopyranoside is an enzyme substrate used to detect lacZ activity and hence the presence of β-galactosidase.
Biochem/physiol Actions
β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.
Preparation Note
A stock solution can be prepared in molecular biology grade water at a concentration of 3 mg/ml. Alternatively, a stock solution of approximately 20.5 mg/ml is prepared in 100 mM sodium phosphate buffer (pH 7.3). Gentle warming may be required to completely dissolve the product.
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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