T2507

Triosephosphate Isomerase from baker′s yeast (S. cerevisiae)

Name: Triosephosphate Isomerase from baker′s yeast (<I>S. cerevisiae</I>)
Brand: SIGMA

Type I, ammonium sulfate suspension, ~10,000 units/mg protein

Synonym(s):

D-Glyceraldehyde-3-phosphate ketol-isomerase, TPI

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About This Item

CAS Number:

9023-78-3

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-774-2

MDL number:

MFCD00082080

EC Number:

5.3.1.1 (BRENDA, IUBMB)

Product Name

Triosephosphate Isomerase from baker′s yeast (S. cerevisiae), Type I, ammonium sulfate suspension, ~10,000 units/mg protein

type

Type I

form

ammonium sulfate suspension

specific activity

~10,000 units/mg protein

mol wt

53 kDa by equilibrium centrifugation

foreign activity

pyruvate kinase, lactic dehydrogenase, 3-phosphoglyceric phosphokinase, phosphoglucose isomerase, α-glycerophosphate dehydrogenase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase <0.01%

storage temp.

2-8°C

Quality Level

200

Related Categories

Phosphates

Analysis Note

Protein determined by biuret.

Application

Triosephosphate isomerase has been used in a study to assess differential expression of fourteen proteins of uveal melanoma. Triosephosphate isomerase has also been used in a study to investigate the use of sigmoid pH gradients in free-flow isoelectric focusing of human endothelial cell proteins.

Other Notes

One unit will convert 1.0 μmole D-glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 at 25 °C.

Since the assay of this enzyme seems to be sensitive to minor variables, packages are dispensed per mg protein rather than in units.

Physical form

Crystalline suspension in 2.7 M (NH₄)₂SO₄, 0.5 mM EDTA, pH 6.5

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number

036H8025

060H8110

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The use of sigmoid pH gradients in free-flow isoelectric focusing of human endothelial cell proteins.

Xiao Ma et al.

Electrophoresis, 33(9-10), 1349-1355 (2012-06-01)

Prefractionation of proteins enhances the resolution of proteome analysis of whole cells. Free-flow electrophoresis (FFE) provides a useful step in various prefractionation protocols, since matrix-free isoelectric focusing (FF-IEF) performed in this machine enables the enrichment of large, easily absorbable, sensitive

Differential expression of fourteen proteins between uveal melanoma from patients who subsequently developed distant metastases versus those who did Not.

Annett Linge et al.

Investigative ophthalmology & visual science, 53(8), 4634-4643 (2012-05-10)

To compare the proteomic profiles of two categories of primary uveal melanoma tissue samples; those from patients who have subsequently developed metastatic disease and those who have not. Two-dimensional difference gel electrophoresis (2D DIGE) was performed on 25 uveal melanoma

Knockout of TIGAR enhances myocardial phosphofructokinase activity and preserves diastolic function in heart failure.

Xiaochen He et al.

Journal of cellular physiology, 237(8), 3317-3327 (2022-05-28)

Hypertension is an important risk factor in the pathogenesis of diastolic dysfunction. Growing evidence indicates that glucose metabolism plays an essential role in diastolic dysfunction. TP53-induced glycolysis and apoptosis regulator (TIGAR) has been shown to regulate glucose metabolism and heart

Development of a quantitative PCR (qPCR) for Giardia and analysis of the prevalence, cyst shedding and genotypes of Giardia present in sheep across four states in Australia.

Rongchang Yang et al.

Experimental parasitology, 137, 46-52 (2013-12-21)

A novel quantitative PCR (qPCR) for Giardia at the glutamate dehydrogenase (gdh) locus was developed and validated. The qPCR was used to screen a total of 3412 lamb faecal samples collected from approximately 1189 lambs at three sampling periods (weaning

Precision is essential for efficient catalysis in an evolved Kemp eliminase.

Rebecca Blomberg et al.

Nature, 503(7476), 418-421 (2013-10-18)

Linus Pauling established the conceptual framework for understanding and mimicking enzymes more than six decades ago. The notion that enzymes selectively stabilize the rate-limiting transition state of the catalysed reaction relative to the bound ground state reduces the problem of

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