W1878
Williams′ Medium E
With sodium bicarbonate, without ʟ-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
Williams’ E medium
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
Product Name
Williams′ Medium E, With sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
sodium pyruvate: 0.025 g/L
phenol red: no
glucose: 2.0 g/L (Dextro)
NaHCO3: 2.2 g/L
L-glutamine: no
shipped in
ambient
storage temp.
2-8°C
Quality Level
General description
Williams′ Medium E was developed by Williams and Gunn as a modification to Williams′ Medium D. The medium is formulated to support long-term cell culture of adult liver epithelial cells. It is a modified Minimum Essential Medium (MEM) with altered glucose and amino acid content.
Other Notes
Phenol red has been shown to interfere with the growth of some cells at low or cloning densities. Use this version of William's E when working with stem cells or cells at low densities.
Preparation Note
Supplement with 0.292 g/L L-glutamine.
Application
Williams′ Medium E has been used in the culturing of human hepatocellular carcinoma (HCC) cells and hepatic cells.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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