203806
5-Bromo-2′-deoxyuridine
Thymidine analog. Useful for the study of DNA synthesis where it is incorporated into DNA in place of thymidine.
Synonym(s):
5-Bromo-2′-deoxyuridine, 5-BrdU
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About This Item
Assay:
≥98% (HPLC)
Form:
solid
Solubility:
water: 10 mg/mL, 0.1 M NaOH: 50 mg/mL, DMSO: 50 mg/mL
Storage temp.:
15-25°C
description
RTECS - YU7350000
Quality Segment
assay
≥98% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
color
white
solubility
water: 10 mg/mL, 0.1 M NaOH: 50 mg/mL, DMSO: 50 mg/mL
shipped in
ambient
storage temp.
15-25°C
SMILES string
Brc1[c]([nH][c]([n](c1)C2OC(C(C2)O)CO)=O)=O
InChI
1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)
InChI key
WOVKYSAHUYNSMH-UHFFFAOYSA-N
General description
Thymidine analog. Used for the study of DNA synthesis where it is incorporated into DNA in place of thymidine. Can be used both in vitro and in vivo to label DNA. The resulting DNA can be used in conjuction with BrdU (Ab-2) Monoclonal Antibody (Cat. No. NA20) or BrdU Immunohistochemical System (Cat. No. HCS24) for the immunohistochemical analysis of cell proliferation.
Thymidine analog. Useful for the study of DNA synthesis where it is incorporated into DNA in place of thymidine. Can be used both in vitro and in vivo to label DNA. The resulting DNA can be used in conjunction with BrdU (Ab-2) Monoclonal Antibody (Cat. No. NA20 ) for the immunohistochemical analysis of cell proliferation.
Preparation Note
Following reconstitution, sterilize by filtration through a 0.22 µm filter, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Garabedian, E.M., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 15382.
Cattoretti, G., et al. 1993. J. Pathol. 171, 83.
Fukuda, K., et al. 1990. Anal. Quant. Cytol. Histol. 12, 135.
Cattoretti, G., et al. 1993. J. Pathol. 171, 83.
Fukuda, K., et al. 1990. Anal. Quant. Cytol. Histol. 12, 135.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Harmful & Carcinogenic / Teratogenic (E)
signalword
Danger
hcodes
Hazard Classifications
Muta. 1B - Repr. 2
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 2
Certificates of Analysis (COA)
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