A9594
Monoclonal ANTI-FLAG® M2-Cy3 antibody, Mouse monoclonal
clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)
Synonym(s):
Monoclonal ANTI-FLAG® M2, Anti-ddddk, Anti-dykddddk
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About This Item
NACRES:
NA.32
UNSPSC Code:
41106514
Conjugate:
CY3 conjugate
Clone:
M2, monoclonal
Application:
IF (d)
Citations:
42
shipped in
dry ice
biological source
mouse
conjugate
CY3 conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
M2, monoclonal
form
buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)
species reactivity
all
concentration
~1 mg/mL
technique(s)
direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone
isotype
IgG1
immunogen sequence
DYKDDDDK
storage temp.
−20°C
Quality Level
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General description
Monoclonal ANTI-FLAG M2-Cy3 (mouse IgG) antibody is covalently conjugated to cyanine dye Cy3. The antibody conjugate binds to FLAG fusion proteins, and will recognize the FLAG sequence at the N-terminus, Met-N-terminus, or C-terminus of FLAG fusion proteins.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
For simple, one-step detection by immunocytochemistry. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies might cause cross-reactivity.
Learn more product details in our FLAG® application portal.
Learn more product details in our FLAG® application portal.
Physical form
Solution in phosphate buffered saline plus 1% BSA and preservative
Preparation Note
Dilute the antibody in Tris buffered saline (TBS): 0.05 M Tris,pH 7.4, with 0.15 M NaCl.
Other Notes
Suggested concentration of 1-10 mg/ml for immunocytochemistry.
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Cy3 is a trademark of Cytiva
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Journal of virology, 88(9), 4943-4952 (2014-02-21)
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Q Jin et al.
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Thomas L Edwards et al.
The Biochemical journal, 423(1), 31-39 (2009-07-08)
The HSPs (hereditary spastic paraplegias) are genetic conditions in which there is distal degeneration of the longest axons of the corticospinal tract, resulting in spastic paralysis of the legs. The gene encoding spartin is mutated in Troyer syndrome, an HSP
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