F4135
Fetal Bovine Serum
USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma
Synonym(s):
HI FBS, HI FCS, HI sera, HI serum, FBS, FCS, sera, serum
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About This Item
Biological source:
bovine fetus
Origin:
USA origin
Sterility:
sterile-filtered
Shipped in:
dry ice
biological source
bovine fetus
sterility
sterile-filtered
product line
FBS Classic
composition
Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL
origin
USA origin
technique(s)
cell culture | hybridoma: suitable, cell culture | insect: suitable, cell culture | mammalian: suitable
impurities
≤10 EU/mL endotoxin
shipped in
dry ice
storage temp.
−20°C
Quality Level
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General description
Fetal Bovine Serum (FBS) remains a popular media supplement as it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and aids in proliferation. It also protects cells from oxidative damage and apoptosis. FBS blocks reactive oxygen species generation, which otherwise is not eliminated in serum-free media (SFM) systems.
Application
FBS sourced in the United States is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture.
Fetal Bovine Serum has been used:
Fetal Bovine Serum has been used:
- as a component of Dulbecco′s Modified Eagles Media (DME, DMEM), Eagle′s Minimum Essential Medium (EMEM), Roswell Park Memorial Institute medium (RPMI-1640), α-modified eagle medium (αMEM) (MEM) and Leibovitz L-15 medium
- as a component of blocking medium for treating brainstem slices
- to improve attachment of dermal explants
Preparation Note
Prepared by heating at 56°C for 30 minutes.
This product is prepared by heating at 56°C for 30 minutes. Heating serum to 56°C for 30 minutes inactivates the complement system for immunoassays. Heat activation also inactivates other undetermined inhibitors of cell growth in culture.
Analysis Note
- Endotoxin and hemoglobin tested
- Tested for the presence of bacteria, virus, and mycoplasma
- Triple filtered with 0.1 micron membrane under aseptic conditions
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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Antileishmanial activity study and theoretical calculations for 4-amino-1, 2, 4-triazole derivatives
Suleymanouglu N, et al.
Journal of Molecular Structure, 1144, 80-86 (2017)
Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts: an in vitro approach to bovine digital dermatitis
Akkoc A, et al.
Journal of Applied Animal Research, 46, 445-450 (2018)
Deven Patel et al.
The Journal of biological chemistry, 291(17), 9322-9329 (2016-02-28)
During G1-phase of the cell cycle, normal cells respond first to growth factors that indicate that it is appropriate to divide and then later in G1 to the presence of nutrients that indicate sufficient raw material to generate two daughter
Ming Guan et al.
American journal of translational research, 11(3), 1343-1358 (2019-04-12)
Aging and exposure to noise or ototoxic drugs are major causes of hair cell death leading to human hearing loss, and many agents have been developed to protect hair cells from apoptosis. Fetal bovine serum (FBS) is a fundamental ingredient
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