Insulin-transferrin-sodium selenite media supplement

Insulin-transferrin-sodium selenite media supplement supports in vitro growth and maturation of follicles from oocyte and is routinely used in in vitro fertilization methods.

Serum-free and reduced serum formulations often require purified forms of the factors normally supplied by serum that are suitable for in vitro growth and differentiation studies. These important factors include insulin, transferrin, selenium, pyruvate, and ethanolamine.

INSULIN is a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe observed mitogenic effect to this property.

TRANSFERRIN is an iron-transport protein. Iron is an essential trace element, but can be toxic in the free form. To nourish cells in culture, it is supplied bound to transferrin in serum.

SELENIUM is an essential trace element normally provided by Serum.

Nutritional studies indicate that the supplement components described are utilized by most mammalian cells. They enhance cell proliferation and decrease the serum requirement of many cell types. When the provided supplement is used with 2 to 4 percent serum, proliferation is reported to be similar to medium supplemented with 10 percent serum.

Insulin-transferrin-sodium selenite media supplement has been used as:

• medium 199 (M199) media supplement for cardiac myocytes culture
• Roswell Park Memorial Institute (RPMI) 1640 medium component for pluripotent stem cell (iPSC) culture
• Dulbecco′s Modified Eagle Medium (DMEM) supplement in ID8 ovarian carcinoma cells

Each vial sufficient to prepare 5 liters of medium.

Each vial contains: ≥21 mg recombinant human insulin; ≥19 mg human transferrin (substantially iron free), and 25 μg sodium selenite.

ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.