M5519
Murashige and Skoog Basal Medium
powder, suitable for plant cell culture
Synonym(s):
MS0 Medium, MSO Medium, MS Basal Medium
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About This Item
form
powder
Quality Segment
technique(s)
cell culture | plant: suitable
application(s)
agriculture
storage temp.
2-8°C
General description
Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. Important growth regulators frequently added to M&S include IAA (auxin/morphogen) and the Kinetin (cytokinin/cell division promoter).
Application
Murashige and Skoog medium has been used in plant tissue culture medium for plant transformation study.
Preparation Note
Formulated to contain 4.4 grams of powder per liter of medium.
Murashige and Skoog medium can be reconstituted from powder or by combining products that are major components of complete M&S medium, such as macronutrient mixtures and vitamin mixtures. Murashige and Skoog medium (M5519) contains the micronutrients and vitamins of the original classic formulation. It can be supplemented with sucrose, agar, auxins (IAA) and cytokinins (Kinetin) to generate a complete medium for growth plant tissue culture.
With the macro- and micronutrients, and vitamins as described by Murashige and Skoog (1962).
Media Formulation
Media Formulation
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Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Ox. Sol. 3
Storage Class
5.1B - Oxidizing hazardous materials
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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