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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ELISA, ICC, IHC, IP, WB
Citations:
43
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
chicken, zebrafish, human, bovine, mouse, rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... NRGN(4900)
General description
7.5 kDa
Immunogen
Recombinant rat Neurogranin (complete sequence).
Application
Immunoblot: >1:500-1:000
Immunohistochemistry: >1:1,000 on 4% paraformaldehyde / PBS fixed or 3% Paraformaldehyde / 0.1% Gluteraldehyde / PBS fixed tissue sections.
Immunocytochemistry: >1:1,000.
Immunoprecipitation: >1:100
ELISA: >1:2,000
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: >1:1,000 on 4% paraformaldehyde / PBS fixed or 3% Paraformaldehyde / 0.1% Gluteraldehyde / PBS fixed tissue sections.
Immunocytochemistry: >1:1,000.
Immunoprecipitation: >1:100
ELISA: >1:2,000
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
This Anti-Neurogranin Antibody is validated for use in ELISA, IC, IH, IP, WB for the detection of Neurogranin.
Biochem/physiol Actions
Recognizes Neurogranin.
Physical form
Ammonium Sulfate Precipitation
Format: Purified
Purified immunoglobulin. Purified by ammonium sulfate precipitation. Liquid in PBS with 0.02% sodium azide as a preservative.
Preparation Note
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Brain tissue
Brain tissue
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Related Content
Elisabetta Cesana et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(30), 12430-12446 (2013-07-26)
The function of inhibitory interneurons within brain microcircuits depends critically on the nature and properties of their excitatory synaptic drive. Golgi cells (GoCs) of the cerebellum inhibit cerebellar granule cells (GrCs) and are driven both by feedforward mossy fiber (mf)
Sandra L Wilson et al.
Cerebellum (London, England), 10(3), 356-372 (2011-03-25)
The cerebellum is a highly organized structure partitioned into lobules along the anterior-posterior (A-P) axis and into striped molecular domains along the medial-lateral (M-L) axis. The Engrailed (En) homeobox genes are required for patterning the morphological and molecular domains along
Chiara Rolando et al.
The European journal of neuroscience, 31(8), 1340-1351 (2010-04-14)
Stem or progenitor cells acquire specific regional identities during early ontogenesis. Nonetheless, there is evidence that cells heterotopically transplanted to neurogenic regions of the developing or mature central nervous system may switch their fate to adopt host-specific phenotypes. Here, we
Global Trade Item Number
| SKU | GTIN |
|---|---|
| AB5620 | 04053252744280 |