Monoclonal Anti-Calmodulin antibody produced in mouse

Calmodulin belongs to a family of structurally homologous Ca²⁺-binding proteins. CALM1 gene is located on human chromosome 14q32.11.

Calmodulin is a highly conserved 17kDa calcium binding protein found in all eukaryotic cells. It is a multifunctional molecule. Monoclonal anti-Calmodulin (mouse IgG1 isotype) is derived from the 6D4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with calmodulin, purified from Dictyostelium discoideum and conjugated to KLH.

preparation of purified calmodulin from Dictyostelium discoideum conjugated to KLH.

Monoclonal Anti-Calmodulin antibody produced in mouse has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunoblot
• dot blot
• immunocytochemistry
• immunoprecipitation
• immunofluorescence

Calmodulin is a ubiquitous molecule which can bind up to 4 calcium ions, regulating and mediating a wide variety of biochemical processes.

The antibody recognizes native and SDS-denatured calmodulin.

These antibodies are directed against different epitopes within the calmodulin molecule.

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