P3326
Platelet-Derived Growth Factor-AB human
≥97% (SDS-PAGE and N-terminal analysis), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym(s):
PDGF-AB
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About This Item
Biological source:
human
Recombinant:
expressed in E. coli
Assay:
≥97% (SDS-PAGE and N-terminal analysis)
Form:
lyophilized powder
Mol wt:
27-31 kDa
Impurities:
≤1 EU/μg
Product Name
Platelet-Derived Growth Factor-AB human, PDGF-AB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
biological source
human
Quality Level
recombinant
expressed in E. coli
assay
≥97% (SDS-PAGE and N-terminal analysis)
form
lyophilized powder
potency
10-100 ng/mL ED50/EC50
quality
endotoxin tested
mol wt
27-31 kDa
packaging
pkg of 10 μg, pkg of 50 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1 EU/μg
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... PDGFA(5154), PDGFB(5155)
General description
Platelet-Derived Growth Factor (PDGF) is a cationic polypeptide and a constituent of α-granules. PDGF is composed of chain A and B linked together by a disulphide bridge. PDGF heterodimers occurs as PDGF-AB and homodimers exists as FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD). The PDGFB gene is localized to pericytes in the brain and is mapped to human chromosome 7p22.3.
Application
Platelet-Derived Growth Factor-AB human has been used in luciferase reporter assay.
Biochem/physiol Actions
Cellular actions of platelet-derived growth factor (PDGF) include chemoattraction and activation of neutrophils, monocytes, and fibroblasts. PDGF appears to play a crucial role in the cellular response to tissue injury and is critically involved in the pathological progression of atherosclerosis.
PDGF stimulates fibroblast proliferation and extracellular matrix production in order to facilitate tissue repair. PDGF-AB isomer is found to be associated with angiogenesis and proliferation. Loss of PDGFB gene function induces microvascular calcification due to age-dependent phenotypical changes.
PDGF stimulates fibroblast proliferation and extracellular matrix production in order to facilitate tissue repair. PDGF-AB isomer is found to be associated with angiogenesis and proliferation. Loss of PDGFB gene function induces microvascular calcification due to age-dependent phenotypical changes.
Physical form
Lyophilized from a 0.2 μm filtered solution in 40% acetonitrile and 0.1% trifluoroacetic acid.
Analysis Note
The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.
Other Notes
Heterodimer of the A and B chains of PDGF disulfide linked.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Related Content
Instructions
Injury induces in vivo expression of platelet-derived growth factor (PDGF) and PDGF receptor mRNAs in skin epithelial cells and PDGF mRNA in connective tissue fibroblasts.
Antoniades HN, et al.
Proceedings of the National Academy of Sciences of the USA, 88.2 , 565-569 (1991)
Increased levels of platelet-derived growth factor in vitreous fluid of patients with proliferative diabetic retinopathy.
Freyberger H, et al.
Experimental and Clinical Endocrinology & Diabetes, 108.02, 106-109 (2000)
Clinical Findings Associated with a De Novo Partial Trisomy 10p11. 22p15. 3 and Monosomy 7p22. 3 Detected by Chromosomal Microarray Analysis.
Kohannim O, et al.
Case Reports in Genetics, 2011 (2011)