A0410
Aminoallyl-dUTP sodium salt
≥85%, lyophilized powder
Synonym(s):
5-[3-Aminoallyl]-2′-deoxyuridine 5′-triphosphate, AA-dUTP
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About This Item
Empirical Formula (Hill Notation):
C12H20N3O14P3 · xNa+
CAS Number:
Molecular Weight:
523.22 (free acid basis)
MDL number:
UNSPSC Code:
41106305
NACRES:
NA.51
Product Name
Aminoallyl-dUTP sodium salt, ≥85%, lyophilized powder
InChI key
YWKTUHBZWBRYHX-TYYBGVCCSA-N
SMILES string
[P](=O)(O[P](=O)(OCC1OC(CC1O)N2C=C(C(=O)NC2=O)\C=C\CN)O)(O[P](=O)(O)O)O.N
assay
≥85%
form
lyophilized powder
shipped in
dry ice
storage temp.
−70°C
Quality Level
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Application
Aminoallyl-dUTP may be used for indirect stable labeling of first-strand cDNA synthesis via polymerase chain reaction (PCR). Aminoallyl-dUTP functions as a reactive site for subsequent labeling by reactive fluorescent probes. Aminoallyl-dUTP may also be useful in the construction of reactive DNA scaffolds.
Aminoallyl-dUTP sodium salt has been used:
- to add to the deoxynucleoside triphosphate mix for complementary DNA (cDNA) synthesis
- for the generation of chromatin immunoprecipitation (ChIP) probes
- in the reverse transcription of RNA for the synthesis of fluorescent labelled probes
Biochem/physiol Actions
Aminoallyl-dUTP sodium salt/5-(3-aminoallyl)-2′-deoxyuridine 5′-triphosphate (aa-dUTP) have been used mainly in two-step labeling approach for the synthesis of nucleic acid hybridisation probe.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Lorenzo Berti et al.
Nanotechnology, 20(23), 235101-235101 (2009-05-19)
Functional DNA scaffolds can be defined as DNA-based structures comprising chemical moieties facilitating and guiding the immobilization of additional nanocomponents. Due to the limited reactivity of DNA there is currently a need to develop rapid routes to expand its chemical
Miguel A Matilla et al.
Genome biology, 8(9), R179-R179 (2007-09-06)
Mutualistic interactions less well known than those between rhizobia and legumes are commonly found between plants and bacteria, frequently pseudomonads, which colonize roots and adjacent soil areas (the rhizosphere). A global analysis of Pseudomonas putida genes expressed during their interaction
Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling
Cox W G and Singer V L
Biotechniques, 36(1), 114-122 (2004)
Kevin K Fuller et al.
mBio, 4(2) (2013-03-28)
Light is a pervasive environmental factor that regulates development, stress resistance, and even virulence in numerous fungal species. Though much research has focused on signaling pathways in Aspergillus fumigatus, an understanding of how this pathogen responds to light is lacking.
Chromatin immunoprecipitation (ChIP) on chip experiments uncover a widespread distribution of NF-Y binding CCAAT sites outside of core promoters
Testa A, et al.
The Journal of Biological Chemistry, 280(14), 13606-13615 (2005)
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