D4154

Plasmid DNA from E. coli RRI

Name: Plasmid DNA from <I>E. coli</I> RRI
Brand: SIGMA

pUC18, buffered aqueous solution

Select a Size

All Photos(1)

About This Item

CAS Number:

9007-49-2

UNSPSC Code:

41105600

NACRES:

NA.85

MDL number:

MFCD00166896

Product Name

Plasmid DNA from E. coli RRI, pUC18, buffered aqueous solution

SMILES string

[P](=O)(OC2C(OC(C2)N)CO[P](=O)(OC3C(OC(C3)N)CO)O)(OCC1OC(CC1O)N)O

InChI

1S/C15H31N3O13P2/c16-13-1-7(20)11(28-13)5-25-32(21,22)31-9-3-15(18)29-12(9)6-26-33(23,24)30-8-2-14(17)27-10(8)4-19/h7-15,19-20H,1-6,16-18H2,(H,21,22)(H,23,24)

InChI key

AWBASQCACWFTGD-UHFFFAOYSA-N

biological source

Escherichia coli (RRI)

grade

Molecular Biology

form

buffered aqueous solution

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

Looking for similar products? Visit Product Comparison Guide

Application

Plasmid DNA from Escherichia coli RRI has been used in polymerase chain reaction (PCR).

Plasmid DNA from E. coli RRI has also been used for in vitro DNA transfection studies.

Biochem/physiol Actions

Foreign DNA inserted at the MCS interrupts the β-galactosidase gene and abolishes the ability to catabolize lactose. Lactose-positive, ampicillin-resistant colonies (host strain containing plasmid) form blue colonies on plates containing ampicillin and X-Gal; lactose-negative, ampicillin-resistant colonies (host strain containing plasmid with foreign DNA inserted at the MCS) form white colonies on this medium.

These plasmids confer ampicillin resistance and complement defects in β-galactosidase in appropriate host strains. The multiple cloning site (MCS) is within the β-galactosidase gene; pUC8 and 9 have nine unique sites within the MCS while pUC18 and 19 have thirteen.
Foreign DNA inserted at the MCS abolishes the ability to catabolize lactose. Lactose-positive, ampicillin-resistant colonies (host strain containing plasmid) form blue colonies on plates containing ampicillin and X-Gal; lactose-negative, ampicillin-resistant colonies (host strain containing plasmid with foreign DNA inserted at the MCS) form white colonies on this medium. The orientations of the MCS regions in the pUC plasmids are analogous to those of the corresponding M13 phage.

General description

Plasmid DNA from Escherichia coli RRI confers ampicillin resistance and complement defects in β-galactosidase in appropriate host strains. The multiple cloning site (MCS) is within the β-galactosidase gene. pUC8 and 9 have nine unique sites within the MCS while pUC18 and 19 have thirteen.

Other Notes

pUC18 Plasmid DNA, 10 μg is supplied at approximately 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Nucleoside phosphocholine amphiphile for in vitro DNA transfection

Moreau L, et al.

Molecular Biosystems, 1(3), 260-264 (2005)

Quantification of beta-human papillomavirus DNA by real-time PCR.

Weissenborn SJ

Nature Protocols, 5(1), 1-13 (2010)

Complete mitochondrial genome of wild aurochs (Bos primigenius) reconstructed from ancient DNA.

J Zeyland et al.

Polish journal of veterinary sciences, 16(2), 265-273 (2013-08-27)

Extinct aurochs (Bos primigenius), accepted as the ancestor of domestic cattle, was one of the largest wild animals inhabiting Europe, Asia and North Africa. The gradual process of aurochs extinction finished in Poland in 1627, were the last recorded aurochs

Temperature and magnetic field effects on electron transport through DNA molecules in a two-dimensional four-channel system.

Yong S Joe et al.

Journal of nanoscience and nanotechnology, 13(6), 3889-3896 (2013-07-19)

We utilize a two-dimensional four-channel DNA model, with a tight-binding (TB) Hamiltonian, and investigate the temperature and the magnetic field dependence of the transport behavior of a short DNA molecule. Random variation of the hopping integrals due to the thermal

A new era in noninvasive prenatal testing.

Stephanie Morain et al.

The New England journal of medicine, 369(6), 499-501 (2013-07-19)

View All Related Papers