F0291

Fibroblast Growth Factor-Basic, human

Name: Fibroblast Growth Factor-Basic, human
Brand: SIGMA

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):

Fibroblast Growth Factor-Basic human, FGF2, bFGF, hBFGF

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About This Item

CAS Number:

106096-93-9

UNSPSC Code:

12352202

NACRES:

NA.77

MDL number:

MFCD00081382

Product Name

hBFGF, FGF-Basic, recombinant, expressed in E. coli, suitable for cell culture

biological source

human

recombinant

expressed in E. coli

assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

≤-0.6 ng/mL

mol wt

16.0 kDa

packaging

pkg of 4X25 μg
pkg of 25 μg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤1.00 EU/μg

color

white to faint yellow cast

solubility

water: soluble 0.025 mg, clear, colorless to faintly yellow

UniProt accession no.

P09038

storage temp.

−20°C

Quality Level

200

Gene Information

human ... FGF2 (2247)

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Analysis Note

The bioactivity of FGF-Basic was measured in a fluorometric assay using the redox sensitive dye, Resazurin.

Application

Fibroblast growth factor-basic (FGF-Basic) is required for the maintenance of human embryonic stem cells in culture, and various other stem cell lines, such as mesenchymal stem (stroma) cells (MSC).

General description

Fibroblast growth factor-basic (FGF-Basic) is found in basement membranes and sub-endothelial extracellular matrix. FGF-Basic is induced early in the development of the embryo where it is necessary (but not sufficient) for pluripotency and self-renewal of embryonic stem cells (ESC) and lineage defined stem cells; wherein it functions to support proliferation and inhibit differentiation. Self-renewal of hESC depends upon activation of the Activin/Nodal/Smad2,3 branch and suppression of the BMP/GDF/Smad5 branch of the TGFβ-family cell signaling program. FGF-Basic is a competence factor for Nodal support of pluripotency. Activin-A is necessary and sufficient to maintain stemness. It induces the expression of both Nodal and FGF-Basic in human ES cells.

Physical form

Lyophilized from 0.2 μm filtered solution in 20 mM Tris and 1 M NaCl, pH 7.0, containing 50 μg bovine serum albumin per 1 μg BFGF.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Adhesion profile and differentiation capacity of human adipose tissue derived mesenchymal stem cells grown on metal ion (Zn, Ag and Cu) doped hydroxyapatite nano-coated surfaces.

R Beklem Bostancioglu et al.

Colloids and surfaces. B, Biointerfaces, 155, 415-428 (2017-05-02)

Accelerated Mesenchymal Stem Cells (MSCs) condensation and robust MSC-matrix and MSC-MSC interactions on nano-surfaces may provide critical factors contributing to such events, likely through the orchestrated signal cascades and cellular events modulated by the extracellular matrix. In this study, human

Activin A maintains self-renewal and regulates fibroblast growth factor, Wnt, and bone morphogenic protein pathways in human embryonic stem cells.

Lei Xiao et al.

Stem cells (Dayton, Ohio), 24(6), 1476-1486 (2006-02-04)

Human embryonic stem cells (hESCs) self-renew indefinitely while maintaining pluripotency. The molecular mechanism underlying hESCs self-renewal and pluripotency is poorly understood. To identify the signaling pathway molecules that maintain the proliferation of hESCs, we performed a microarray analysis comparing an

Basic fibroblast growth factor supports undifferentiated human embryonic stem cell growth without conditioned medium.

Chunhui Xu et al.

Stem cells (Dayton, Ohio), 23(3), 315-323 (2005-03-08)

Previous studies have shown that prolonged propagation of undifferentiated human embryonic stem cells (hESCs) requires conditioned medium from mouse embryonic feeders (MEF-CM) as well as matrix components. Because hESCs express growth factor receptors, including those for basic fibroblast growth factor

Feeder-free growth of undifferentiated human embryonic stem cells.

C Xu et al.

Nature biotechnology, 19(10), 971-974 (2001-10-03)

Previous studies have shown that maintenance of undifferentiated human embryonic stem (hES) cells requires culture on mouse embryonic fibroblast (MEF) feeders. Here we demonstrate a successful feeder-free hES culture system in which undifferentiated cells can be maintained for at least

Structural characterization and biological functions of fibroblast growth factor.

D Gospodarowicz et al.

Endocrine reviews, 8(2), 95-114 (1987-05-01)

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