M7167

M2 medium

Name: M2 medium
Brand: SIGMA

With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for mouse embryo cell culture

Synonym(s):

Cell Culture M2, Cell Culture Medium, M2 Medium Solution

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

MDL number:

MFCD00283761

Product Name

M2 medium, With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for mouse embryo cell culture

sterility

sterile-filtered

form

liquid

storage condition

protect from light

technique(s)

cell culture | embryo: suitable

impurities

endotoxin, tested

components

HEPES: 5.42726 g/L
NaHCO₃: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L

shipped in

ambient

storage temp.

2-8°C

Quality Level

300

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Related Categories

Cell Culture & Analysis

Cell Culture Media & Buffers

Cell Lines & Specialty Cell Culture

Classical Media & Buffers

Molecular Biology & Functional Genomics

Analysis Note

Presence of particulates in solution will not affect product′s performance.

This product is tested for its ability to support the development of one-cell mouse embryos to expanded blastocysts. Minimum requirement is 80% development to blastocyst.

Application

Recommended for manipulation of mouse embryos at ambient temperature. M2 and M16 Medium are common media for in vitro culture of preimplantation stage embryos. It is a modified Krebs-Ringer bicarbonate solution, which is very similar to Whitten′s Medium. M16 contains pyruvate and lactate as energy sources since preimplantation embryos do not utilize glucose efficiently.

M2 Medium is a further modification of M16 that substitutes HEPES buffer in place of some of the bicarbonate. M2 is used for collecting and handling embryos for prolonged periods outside a CO2 incubator.

Preparation Note

Supplement with 0.06 g/L potassium penicillin-G and 0.05 g/L streptomycin sulfate.

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Prenatal Exposure to Environmentally-Relevant Contaminants Perturbs Male Reproductive Parameters Across Multiple Generations that are Partially Protected by Folic Acid Supplementation.

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Scientific reports, 9(1), 13829-13829 (2019-09-27)

The paternal environment is thought to influence sperm quality and future progeny may also be impacted. We hypothesized that prenatal exposure to environmentally-relevant contaminants impairs male reproduction, altering embryo gene expression over multiple generations. Folic acid (FA) can improve sperm

Maternal DCAF2 is crucial for maintenance of genome stability during the first cell cycle in mice.

Yi-Wen Xu et al.

Journal of cell science, 130(19), 3297-3307 (2017-08-19)

Precise regulation of DNA replication and genome integrity is crucial for gametogenesis and early embryogenesis. Cullin ring-finger ubiquitin ligase 4 (CRL4) has multiple functions in the maintenance of germ cell survival, oocyte meiotic maturation, and maternal-zygotic transition in mammals. DDB1-cullin-4-associated

Mapping of histone-binding sites in histone replacement-completed spermatozoa.

Keisuke Yoshida et al.

Nature communications, 9(1), 3885-3885 (2018-09-27)

The majority of histones are replaced by protamines during spermatogenesis, but small amounts are retained in mammalian spermatozoa. Since nucleosomes in spermatozoa influence epigenetic inheritance, it is important to know how histones are distributed in the sperm genome. Conflicting data

Oncostatin M expression in the mouse uterus during early pregnancy promotes embryo implantation and decidualization.

Tao Fu et al.

FEBS letters, 593(15), 2040-2050 (2019-06-04)

Oncostatin M (OSM) is a member of the interleukin-6 (IL-6) family, which functions in embryo implantation and decidualization. The expression, function and regulation of Osm in mouse uteri during early pregnancy remain unknown. We show that Osm is mainly expressed

Transgenerational transmission of hedonic behaviors and metabolic phenotypes induced by maternal overnutrition.

Gitalee Sarker et al.

Translational psychiatry, 8(1), 195-195 (2018-10-14)

Maternal overnutrition has been associated with increased susceptibility to develop obesity and neurological disorders later in life. Most epidemiological as well as experimental studies have focused on the metabolic consequences across generations following an early developmental nutritional insult. Recently, it

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