U6504

Urea

Name: Urea
Brand: SIGMA

suitable for electrophoresis

Synonym(s):

Carbamide, Carbonyldiamide

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All Photos(3)

About This Item

Linear Formula:

NH₂CONH₂

CAS Number:

57-13-6

Molecular Weight:

60.06

UNSPSC Code:

12352111

NACRES:

NB.22

PubChem Substance ID:

24900435

EC Number:

200-315-5

Beilstein/REAXYS Number:

635724

MDL number:

MFCD00008022

assay:

≥98%

technique(s):

electrophoresis: suitable

Product Name

Urea, suitable for electrophoresis

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

SMILES string

NC(N)=O

assay

≥98%

form

powder or crystals

technique(s)

electrophoresis: suitable

impurities

≤0.005% Ammonia

mp

132-135 °C (lit.)

solubility

H₂O: 100 mg/mL

density

1.335 g/mL at 25 °C (lit.)

cation traces

Fe: ≤5 ppm
Pb: ≤5 ppm

absorption

≤0.06 at 260 at 5 M
≤0.06 at 280 at 5 M

Quality Level

200

Gene Information

human ... CA1(759), CA2(760)
rat ... Ppm1a(24666)

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Application

A chaotropic agent used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis.

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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