X1629

Anti-XPG (C-terminal) antibody produced in rabbit

Name: Anti-XPG (C-terminal) antibody produced in rabbit
Brand: SIGMA

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-COFS3, Anti-ERCC5, Anti-ERCM2, Anti-UVDR, Anti-XPGC

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About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Product Name

Anti-XPG (C-terminal) antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

concentration

~1 mg/mL

technique(s)

indirect immunofluorescence: 2-4 μg/mL using HEK-293T cells expressing recombinant XPG fusion protein, fixed with paraformaldehyde-Triton
western blot: 1-2 μg/mL using whole cell lysates of COS7 cells expressing recombinant XPG fusion protein

UniProt accession no.

P28715

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ERCC5(2073)

Application

Anti-XPG (C-terminal) antibody produced in rabbit has been used in:

staining cells for xeroderma pigmentosum group G (XPG) detection
immunoblotting
immunofluorescence

Biochem/physiol Actions

Xeroderma pigmentosum group G (XPG) helps to maintain genomic stability. It also participates in RNA transcription by interacting with TFIIH, RNA polymerase II and Gadd45a. XPG also participates in nucleotide excision repair (NER) pathway. Mutations in the XPG gene cause the Cockayne syndrome which is characterized by severe growth defects, mental retardation and cachexia.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Anti-XPG (C-terminal) is produced in rabbit using as immunogen a synthetic peptide corresponding to amino acids 1120-1137 of human XPG, conjugated to KLH via an N-terminal added cysteine residue. Xeroderma pigmentosum group G (XPG) is a DNA repair gene, that is mapped to human chromosome13q33. It is a member of the FEN-1 family of structure-specific nucleases and contains two highly conserved nuclease motifs known as the N and I regions separated by a large insertion. This gene spans around 69kb in length and has15 exons. It is highly polymorphic.

Immunogen

synthetic peptide corresponding to amino acids 1120-1137 of human XPG, conjugated to KLH via an N-terminal added cysteine residue.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number

096M4806V

077K4767

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Identification of the XPG region that causes the onset of Cockayne syndrome by using Xpg mutant mice generated by the cDNA-mediated knock-in method

Shiomi N, et al.

Molecular and Cellular Biology, 24(9), 3712-3719 (2004)

Loss of heterozygosity and lack of mutations of the XPG/ERCC5 DNA repair gene at 13q33 in prostate cancer

Hyytinen ER, et al.

Prostate, 41(3), 190-195 (1999)

XPG rs873601 G> A contributes to uterine leiomyoma susceptibility in a Southern Chinese population

Liu Z, et al.

Bioscience Reports, 38(5), BSR20181116-BSR20181116 (2018)

Lineage-specific control of TFIIH by MITF determines transcriptional homeostasis and DNA repair

Seoane M, et al.

Oncogene, 38(19), 3616-3616 (2019)

Domain swapping between FEN-1 and XPG defines regions in XPG that mediate nucleotide excision repair activity and substrate specificity

Hohl M, et al.

Nucleic Acids Research, 35(9), 3053-3063 (2007)

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