Y1375
YPD Broth
NutriSelect® Basic, powder, for fungi, poly bottle of 250 g, poly bottle of 1 kg
Synonym(s):
Yeast Peptone Dextrose Broth
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About This Item
NACRES:
NA.85
UNSPSC Code:
41171607
Manufacturer/tradename:
NutriSelect® Basic
Ph:
6.3-6.7
Form:
powder
Suitability:
fungi, nonselective for Candida spp., nonselective for Pichia spp., nonselective for Saccharomyces spp., nonselective for Zygosaccharomyces spp.
Application(s):
environmental
food and beverages
microbiology
food and beverages
microbiology
sterility
non-sterile
Quality Level
form
powder
composition
Bacteriological peptone, 20 g/L , Glucose, 20 g/L , Yeast extract, 10 g/L
packaging
poly bottle of 1 kg, poly bottle of 250 g
manufacturer/tradename
NutriSelect® Basic
technique(s)
microbiological culture: suitable
color
beige
pH
6.3-6.7
application(s)
environmental
food and beverages
microbiology
suitability
fungi, nonselective for Candida spp., nonselective for Pichia spp., nonselective for Saccharomyces spp., nonselective for Zygosaccharomyces spp.
General description
YPD Broth is a nutritious general liquid growth medium commonly used for the maintenance and propagation of Pichia pastoris, Saccharomyces cerevisiae, and other yeasts. It is a medium used for molecular cloning, protein expression, or as enrichment media. Peptic digest of animal tissue provides nitrogenous nutrients. Yeast extract provides nitrogenous nutrients as well as Vitamin B Complex. Dextrose provides the carbohydrate and energy source to support the growth of S. cerevisiae.
Application
YPD Broth is recommended for the growth of Saccharomyces cerevisiae for molecular biology purposes. This medium supports the growth of most heterotrophic microorganisms but due to their simple composition, they have been adopted as the basal media for the routine cultivation of yeasts. General methods in yeast genetics specify using Yeast Extract Peptone- Dextrose (YPD) medium for cultivating S. cerevisiae and other yeasts.
Preparation Note
1. Suspend 50 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.
2. Autoclave for 15 minutes at 121°C.
50 g per liter
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Runhui Liu et al.
Journal of the American Chemical Society, 136(11), 4333-4342 (2014-03-13)
Fungal infections are a major challenge to human health that is heightened by pathogen resistance to current therapeutic agents. Previously, we were inspired by host-defense peptides to develop nylon-3 polymers (poly-β-peptides) that are toxic toward the fungal pathogen Candida albicans
Veronique Archambault-Leger et al.
Biotechnology for biofuels, 5(1), 49-49 (2012-07-21)
The impact of hydrothermal flowthrough (FT) pretreatment severity on pretreatment and solubilization performance metrics was evaluated for three milled feedstocks (corn stover, bagasse, and poplar) and two conversion systems (simultaneous saccharification and fermentation using yeast and fungal cellulase, and fermentation
Christina Bergonzo et al.
Proteins, 88(9), 1189-1196 (2020-03-18)
ClpS2 is a small protein under development as a probe for selectively recognizing N-terminal amino acids of N-degron peptide fragments. To understand the structural basis of ClpS2 specificity for an N-terminal amino acid, all atom molecular dynamics (MD) simulations were