Anti-Cytochrome P450 Enzyme CYP3A4 Antibody

57 kDa

A synthetic peptide containing the C-terminal sequence of TVSGA from human CYP3A4. The peptide was coupled to KLH.

Anti-Cytochrome P450 Enzyme CYP3A4 Antibody detects level of Cytochrome P450 Enzyme CYP3A4 & has been published & validated for use in IH & WB.

Research Category
Metabolism

Research Sub Category
Enzymes & Biochemistry

Western blot: 1:1000-1:2000: Using microsomal membrane fractions, detects a band at ~54kDa; other bands may be present as well. Antibody has been successfully used to detect CYP3A4 on LICOR fluorescent westerns as well.

Immunohistochemistry: 10% formalin fixed, paraffin embedded tissues 1:200-1:400, microwave-citric acid antigen recovery is suggested.

Optimal working dilutions must be determined by end user.

The rabbit polyclonal serum detects CYP3A4 in humans and rats; by sequence homology of the immunogen used, cross reactivity to CYP3A7, 3A6v4;3A2v1, 3A8, 3A9 may be expected but is untested at this time.

Rabbit antiSerum. Liquid containing no preservative.

Unpurified

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Control
Liver tissue

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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