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NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
3A11, monoclonal
Species reactivity:
human
Application:
—
Citations:
-
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarlebiological source
mouse
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
3A11, monoclonal
form
liquid
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA
Immunoblotting (1-5 µg/mL)
Immunocytochemistry (10 µg/mL)
Paraffin Sections (3 µg/mL))
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... UHRF1(29128)
General description
Purified mouse monoclonal antibody. Recognizes the ~90 kDa UHRF1 protein.
Recognizes the ~90 kDa UHRF1 protein in HeLa cells and human endometrium tissue.
This Anti-UHRF1 Mouse mAb (3A11) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of UHRF1.
Immunogen
A recombinant polypeptide corresponding to amino acides 694-794 of human rUHRF1, expressed as a GST fusion protein
NA
Application



ELISA (see comments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (3 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
HeLa cells, Human endometrium tissue
HeLa cells, Human endometrium tissue
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Mistry H, et al. 2008. Biochem. Biophys. Res. Commun.375 321.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)
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Clase de almacenamiento
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificados de análisis (COA)
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