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Merck

MAB3199Z

Anti-E-Cadherin Antibody, clone 67A4, Azide Free

clone 67A4, Chemicon®, from mouse

Sinónimos:

CD324, MAB3199

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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
67A4, monoclonal
Application:
FACS, IF
Citations:
10

Documentos clave

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biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

67A4, monoclonal

species reactivity

human

packaging

antibody small pack of 25 μg

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable, immunofluorescence: suitable

isotype

IgG1

NCBI accession no.

NM_004360.2

UniProt accession no.

P12830

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... CDH1(999)

Categorías relacionadas

Immunogen

T-47D breast carcinoma line.

Application

Anti-E-Cadherin Antibody, clone 67A4, Azide Free is an antibody against E-Cadherin for use in FC & IF.
Immunoblotting

FACS Analysis

Functional blocking of E-cadherin

Does not work well for immunoprecipitation

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Antibodies against E-cadherin are suitable to characterize epithelial cells. E-Cadherin is involved in adhesion of epithelial cells (mostly homotypic and homophilic). E-cadherin is also expressed on immature erythroid cells. The antibody 67A4 is suitable to study E cadherin expression by flow cytometry. In addition, it blocks the interaction of E-cadherin molecules and therefore is suitable to study E-cadherin-mediated signal transduction.

Physical form

Format: Purified
Liquid in 0.02M PBS with NaCl, pH=7.6, with no preservatives. Protein A purified immunoglobulin.

Analysis Note

Tested

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Michael K Dame et al.
Laboratory investigation; a journal of technical methods and pathology, 94(2), 222-234 (2013-12-25)
In order to advance a culture model of human colonic neoplasia, we developed methods for the isolation and in vitro maintenance of intact colonic crypts from normal human colon tissue and adenomas. Crypts were maintained in three-dimensional Matrigel culture with
Isao Matsuura et al.
The Journal of biological chemistry, 285(3), 1754-1764 (2009-11-19)
Transforming growth factor-beta (TGF-beta) regulates a wide variety of biological activities. It induces potent growth-inhibitory responses in normal cells but promotes migration and invasion of cancer cells. Smads mediate the TGF-beta responses. TGF-beta binding to the cell surface receptors leads
Gracia Y Ng et al.
Investigative ophthalmology & visual science, 54(13), 7933-7942 (2013-11-10)
Shp2 protein tyrosine phosphatase mediates a wide variety of receptor tyrosine kinases (RTK) cell signaling. Herein, we investigate the role of Shp2 in corneal morphogenesis and homeostasis. Shp2 was conditionally knocked out (Shp2(cko)) in Krt14-rtTA;tet-O-Cre;Shp2(f/f) triple transgenic mice administrated with
The use of an in vitro 3D melanoma model to predict in vivo plasmid transfection using electroporation.
Marrero, B; Heller, R
Biomaterials null
Ceri E Stewart et al.
Journal of allergy, 2012, 943982-943982 (2012-01-31)
The aim of the current study was to evaluate primary (human bronchial epithelial cells, HBEC) and non-primary (Calu-3, BEAS-2B, BEAS-2B R1) bronchial epithelial cell culture systems as air-liquid interface- (ALI-) differentiated models for asthma research. Ability to differentiate into goblet
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MOUSE ANTI-HUMAN E-CADHERIN AZIDE-FREE MONOCLONAL ANTIBODY

Número de artículo de comercio global

SKUGTIN
MAB3199Z04053252578342
MAB3199Z-25UG04054839342691

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