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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
67A4, monoclonal
Application:
FACS, IF
Citations:
10
Servicio técnico
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Permítanos ayudarlebiological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
67A4, monoclonal
species reactivity
human
packaging
antibody small pack of 25 μg
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable, immunofluorescence: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CDH1(999)
Immunogen
T-47D breast carcinoma line.
Application
Anti-E-Cadherin Antibody, clone 67A4, Azide Free is an antibody against E-Cadherin for use in FC & IF.
Immunoblotting
FACS Analysis
Functional blocking of E-cadherin
Does not work well for immunoprecipitation
Optimal working dilutions must be determined by end user.
FACS Analysis
Functional blocking of E-cadherin
Does not work well for immunoprecipitation
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Antibodies against E-cadherin are suitable to characterize epithelial cells. E-Cadherin is involved in adhesion of epithelial cells (mostly homotypic and homophilic). E-cadherin is also expressed on immature erythroid cells. The antibody 67A4 is suitable to study E cadherin expression by flow cytometry. In addition, it blocks the interaction of E-cadherin molecules and therefore is suitable to study E-cadherin-mediated signal transduction.
Physical form
Format: Purified
Liquid in 0.02M PBS with NaCl, pH=7.6, with no preservatives. Protein A purified immunoglobulin.
Analysis Note
Tested
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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