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Merck

D1435

Dimetilsulfóxido

meets EP testing specifications, meets USP testing specifications

Sinónimos:

DMSO

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About This Item

Fórmula lineal:
(CH3)2SO
Número CAS:
67-68-5
Peso molecular:
78.13
UNSPSC Code:
12191502
NACRES:
NA.71
PubChem Substance ID:
57654071
EC Number:
200-664-3
Beilstein/REAXYS Number:
506008
MDL number:
MFCD00002089
assay:
≥99.5%
technique(s):
cryopreservation: suitable
bp:
189 °C (lit.)
vapor pressure:
0.42 mmHg ( 20 °C)

Nombre del producto

Dimetilsulfóxido, meets EP testing specifications, meets USP testing specifications

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

SMILES string

CS(C)=O

agency

meets EP testing specifications
meets USP testing specifications

vapor density

2.7 (vs air)

vapor pressure

0.42 mmHg ( 20 °C)

assay

≥99.5%

form

liquid

autoignition temp.

573 °F

expl. lim.

42 %, 63 °F

technique(s)

cryopreservation: suitable

impurities

≤0.1% water (Karl Fischer, USP)
≤0.2% water (Karl Fischer, EP)

color

colorless

refractive index

n20/D 1.478-1.479
n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubility

H2O: miscible (completely)

density

1.100-1.104 g/mL at 25 °C (relative density (EP))
1.10 g/mL (lit.)

UV absorption

λ: 285 nm Amax: ≤0.20
λ: 295 nm Amax: ≤0.20

Quality Level

200

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Disclaimer

Fácilmente superenfriado, vuelve a fundir lentamente a temperatura ambiente. Producto solidificado que puede volver a licuarse por calentamiento a temperatura ambiente sin detrimento del producto.

Application

Dimethyl sulfoxide has been used:
  • for the dissolution of tetramethylrhodamine dye, which is used as fluorophore for fluorescence in- situ hybridization (FISH) experiments
  • as a control in indirect immunofluorescent assay (IFA) of swine testicular (ST) cells
  • for the suspension and separation of buccal cells
  • a polar aprotic solvent used in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs.

Biochem/physiol Actions

Dimethyl sulfoxide (DMSO) is a low molecular weight, less hydrophilic and a freely permeable cryoprotectant. It is a polar aprotic solvent used in polymerase chain reactions (PCR) and as a cryoprotectant and vitrification agent for the preservation of cells, tissues and organs.

Other Notes

For information on dimethyl sulfoxide miscibility, please visit the following link:
Dimethyl Sulfoxide Miscibility/Immiscibility Table

Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 1

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Certificados de análisis (COA)

Lot/Batch Number
SHBT3964
SHBS9604
SHBS9589
SHBS9588
SHBS8130

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Si necesita una versión concreta, puede buscar un certificado específico por el número de lote.

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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

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  1. What is the molarity of DMSO?

    DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.

  2. What is the DMSO dissolved in?

    DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.

  3. Where does DMSO come from?

    DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.

  4. Does DMSO have any synthetic utility?

    DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes.

  5. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  6. Dimethyl sulfoxide (DMSO) should be a liquid.  Why did it arrive as a solid?

    DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.

  7. What is the difference between Dimethyl sulfoxide (DMSO) Products. D1435 and D2438?

    Products D1435 and D2438 both meet the USP specifications. The D2438 is sterile-filtered, but D1435 is not. Furthermore, the D2438 is quality control tested for endotoxin and use-tested for cell freezing.

  8. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  9. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  10. Is Dimethyl sulfoxide a sterile product?

    No, this product is not considered sterile.  Product Nos. D2560 and D2438 are sterile-filtered.

  11. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  12. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Single-molecule mRNA detection and counting in mammalian tissue
Lyubimova A, et al.
Nature Protocols, 8(9), 1743-1743 (2013)
Low dose dimethyl sulfoxide driven gross molecular changes have the potential to interfere with various cellular processes
Tunccer S, et al.
Scientific reports, 8(1), 14828-14828 (2018)
Buccal micronucleus cytome assay: results of an intra-and inter-laboratory scoring comparison
Bolognesi C, et al.
Mutagenesis, 30(4), 545-555 (2015)
Identification of anti-viral activity of the cardenolides, Na+/K+-ATPase inhibitors, against porcine transmissible gastroenteritis virus
Yang CW, et al.
Toxicology and Applied Pharmacology, 332, 129-137 (2017)
Comparison of the effects of different cryoprotectants on stem cells from umbilical cord blood
Chen G, et al.
Stem Cells International, 2016 (2016)
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