CMC0014
BL21(DE3) Chemically Competent Cells
Escherichia coli, rod shaped
Sinónimos:
Competent E. coli cells
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UNSPSC Code:
41106202
NACRES:
NA.85
Biological source:
Escherichia coli
Growth mode:
adherent or suspension
Morphology:
rod shaped
Servicio técnico
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Permítanos ayudarleNombre del producto
BL21(DE3) Chemically Competent Cells, for protein expression
biological source
Escherichia coli
grade
Molecular Biology
growth mode
adherent or suspension
morphology
rod shaped
technique(s)
microbiological culture: suitable
cell transformation
competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg
shipped in
dry ice
storage temp.
−70°C
General description
The BL21(DE3) Chemically Competent strains contain the phage T7 RNA polymerase gene linked to the IPTG-inducible promoter, for use with any expression plasmid containing the T7 promoter.
Genotype
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
Genotype
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
Application
The E. coli strain BL21 (DE3) has been used:
- to express tau 2N4R isoform (aa1–441) and the truncated tau (aa1–421) using pET19b as expression vector.
- BL21(DE3)Chemically Competent Cells has been used for the transformation and expressionof protegrin-1 (PG1) peptides.
- to express 6His-TEVsite-Hs ARF1 (17-181)-Q71L in pHis2 vector.
Features and Benefits
- a transformation efficiency of >1 × 107 cfu/μg.
- economical prices
- offering superior value for everyday protein expression work.
Other Notes
- BL21(DE3) chemically competent cells
- pUC 19 transformation control DNA
- recovery medium for expression
Clase de almacenamiento
10 - Combustible liquids
wgk
WGK 2
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Physiological aggregation of a recombinant enzyme into enzymatically active inclusion bodies could be an excellent strategy to obtain immobilized enzymes for industrial biotransformation processes. However, it is not convenient to recycle "gelatinous masses" of protein inclusion bodies from one reaction
A validated antibody panel for the characterization of tau post-translational modifications.
Ebru E, et al.
Mol. Neurodegener., 12(1), 87-87 (2017)
David B Sauer et al.
eLife, 9 (2020-09-02)
Citrate, α-ketoglutarate and succinate are TCA cycle intermediates that also play essential roles in metabolic signaling and cellular regulation. These di- and tricarboxylates are imported into the cell by the divalent anion sodium symporter (DASS) family of plasma membrane transporters