D3937
DirectLoad™ 1 kb DNA Ladder
ready-to-use marker for DNA electrophoresis
Sinónimos:
1 kb marker, 1kb gel marker, DNA marker, agarose gel electrophoresis marker
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NACRES:
NA.25
UNSPSC Code:
41105335
Servicio técnico
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Permítanos ayudarleQuality Level
form
liquid
usage
100 uses
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
General description
Sigma′s DirectLoad™ 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb, and 2 kb repeats from 6 to 10 kb. For NA electrophoresis, 5 ul of the marker can be loaded directly into a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting.
Application
Suitable for size determination of dsDNA by DNA electrophoresis with either agarose or polyacrylamide gels.
Features and Benefits
- Ready-to-load
- Easy-to-use
- Popular band sizes
Other Notes
Contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb and 2 kb repeats from 6 to 10 kb.
For optimal resolution, the recommended agarose gel concentration is 0.75%.
Sigma′s DirectLoad™ 1kb Ladder is provided in a gel-loading solution of 2.5% Ficoll (Type 400), 0.0125% bromophenol blue, and 0.00625% xylene cyanol.
Legal Information
DirectLoad is a trademark of Sigma-Aldrich Co. LLC
Clase de almacenamiento
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Aron M Geurts et al.
Methods in molecular biology (Clifton, N.J.), 597, 211-225 (2009-12-17)
The genetic dissection of physiological and pathological traits in laboratory model organisms is accelerated by the ability to engineer loss-of-function mutations at investigator-specified loci. This chapter describes the use of zinc-finger nucleases (ZFNs) for the targeted disruption of endogenous rat