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I9392

3-Methylbutanol

BioReagent, ≥98.5%, Molecular Biology

Sinónimos:

3-Methyl-1-butanol, Isoamyl alcohol, Isopentyl alcohol

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Fórmula lineal:
(CH3)2CHCH2CH2OH
Número CAS:
123-51-3
Peso molecular:
88.15
UNSPSC Code:
12352200
NACRES:
NA.52
PubChem Substance ID:
24896157
EC Number:
204-633-5
Beilstein/REAXYS Number:
1718835
MDL number:
MFCD00002934
Assay:
≥98.5%
Grade:
Molecular Biology
Technique(s):
RNA extraction: suitable
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grade

Molecular Biology

Quality Level

200

product line

BioReagent

assay

≥98.5%

form

liquid

technique(s)

RNA extraction: suitable

pH

5.6 (20 °C, 25 g/L)

density

0.809 g/mL at 20 °C (lit.)

suitability

suitable for nucleic acid purification

storage temp.

room temp

SMILES string

CC(C)CCO

InChI

1S/C5H12O/c1-5(2)3-4-6/h5-6H,3-4H2,1-2H3

InChI key

PHTQWCKDNZKARW-UHFFFAOYSA-N

General description

3-methylbutanol (isoamyl alcohol) is a clear, colorless alcohol commonly used as an organic solvent . It can also be used in phenol-chloroform nucleic acid extraction and purification protocols, where it inhibits RNase activity and reduces foaming. Isoamyl alcohol can also be used for the removal of ethidium bromide from DNA purified by CsCl gradient ultracentrifugation.

Application

3-Methylbutanol (isoamyl alcohol) in combination with phenol and chloroform is used in extraction of RNA. It was used in the synthesis of oligo-RNAs using photocaged adenosine molecules.
3-Methylbutanol has been used as a component of phenol/chloroform/ isoamyl alcohol solution for DNA extraction from blood and from absorbing substrata based forensic samples and biofilms.

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Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Clase de almacenamiento

3 - Flammable liquids

wgk

WGK 1

flash_point_f

110.3 °F - closed cup

flash_point_c

43.5 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificados de análisis (COA)

Lot/Batch Number
SHBS6029
SHBP7087
SHBL4025
SHBK7144
SHBK6074

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Contenido relacionado

Product Information Sheet - I9392

Product Information Sheet

DNA extraction from blood and forensic samples
Guidelines for Molecular Analysis in Archive Tissues, 45-54 (2011)
Section 1 Isolation of nucleic acids
Biofouling Methods, 88-88 (2014)
Steven G Chaulk et al.
Nature protocols, 2(5), 1052-1058 (2007-06-05)
This protocol describes a general method for the preparation of RNAs in which the reactivity or hydrogen-bonding properties of the molecule are modified in a photoreversible fashion by use of a caging strategy. A single caged adenosine, modified at the
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