M5755
MOPS-EDTA-Sodium Acetate (MESA)
powder for RNA electrophoresis buffers
Sinónimos:
MESA Buffer
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NACRES:
NA.25
UNSPSC Code:
12161700
Servicio técnico
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Molecular Biology
Quality Level
product line
BioReagent
form
powder
technique(s)
electrophoresis: suitable
pH
6.8-7.2(reconstituted per directions)
solubility
H2O: soluble, faintly yellow
foreign activity
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
storage temp.
room temp
General description
MOPS – EDTA – Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum resolution.
Application
Suitable for use when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis.
Preparation Note
1x MESA buffer contains:
- 40 mM MOPS
- 10 mM sodium acetate
- 1 mM EDTA (pH 8.3)
Dissolve entire contents of bottle in a final volume of 1L molecular biology grade water. This produces a 10x MESA stock solution that can be further diluted as needed.
Clase de almacenamiento
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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