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Merck

P0501

Phenolphthalein β-D-glucuronide

β-glucuronidase substrate

Sinónimos:

Phenolphthalein β-D-glucosiduronic acid, Phenolphthalein glucuronic acid, Phenolphthalein mono-β-D-glucosiduronic acid

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Fórmula empírica (notación de Hill):
C26H22O10
Número CAS:
Peso molecular:
494.45
UNSPSC Code:
12352204
NACRES:
NA.32
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
68526
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Quality Level

assay

≥97% (TLC)

form

powder

solubility

H2O: soluble 100 mg/mL

storage temp.

−20°C

SMILES string

O[C@@H]1[C@@H](O)[C@@H](O[C@@H]([C@H]1O)C(O)=O)Oc2ccc(cc2)C3(OC(=O)c4ccccc34)c5ccc(O)cc5

InChI

1S/C26H22O10/c27-15-9-5-13(6-10-15)26(18-4-2-1-3-17(18)24(33)36-26)14-7-11-16(12-8-14)34-25-21(30)19(28)20(29)22(35-25)23(31)32/h1-12,19-22,25,27-30H,(H,31,32)/t19-,20-,21+,22-,25+,26?/m0/s1

InChI key

FXJYOZKDDSONLX-XADSOVDISA-N

Application

β-glucuronidase activity was determined by the amount of phenolphthalein released from 0.01 M henolphthalein β-D-glucuronide.
Phenolphthalein β-D-glucuronide has been used as a substrate for the detection of β-D-glucuronidase activity in fecal bacteria.

Biochem/physiol Actions

Phenolphthalein β-D-glucuronide is a chromogenic substrate for β-glucuronidase enzyme and the resultant colored product of the reaction is measured at 552 nm.


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Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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A microtitre plate assay for measuring glycosidase activity
Ball Al, et al.
Journal of Enzyme Inhibition and Medicinal Chemistry, 23(1), 131-135 (2008)
Intestinal health benefits of the water-soluble carbohydrate concentrate of wild grape (Vitis thunbergii) in hamsters
Huang YL, et al.
Journal of Agricultural and Food Chemistry, 60(19), 4854-4858 (2012)
Hisashi Satoh et al.
Water science and technology : a journal of the International Association on Water Pollution Research, 83(6), 1399-1406 (2021-03-27)
Monitoring of Escherichia coli concentrations in river water (RW) is essential to identify fecal pollution of the river. The objective of this study was to assess the suitability of a novel, simple and high throughput method developed in our laboratory